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            • Primary Antibodies ›
            • SIGLEC H Antibodies

            Invitrogen

            SIGLEC H Monoclonal Antibody (eBio440c), APC, eBioscience™

            2 Published Figures
            4 References
            View all (12) SIGLEC H antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # 17-0333-82
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            Price (USD)
            373.00
            100 µg
             
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            Cite SIGLEC H Monoclonal Antibody (eBio440c), APC, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (2)
            Application
            SIGLEC H Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            SIGLEC H Antibody (17-0333-82) in Flow

            C57BL/6 mouse splenocytes were stained with CD45R Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, APC (Product # 17-4031-82) (left) or 0.125 µg of Siglec H Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis.
            View Product

            SIGLEC H Antibody in Flow Cytometry (Flow)
            SIGLEC H Antibody in Flow Cytometry (Flow)
            SIGLEC H Antibody in Flow Cytometry (Flow)

            Product Details

            17-0333-82

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            0.25 µg/test
            View 4 publications 4 publications
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Mouse

            Host/Isotype

            Rat / IgG2b, kappa

            Recommended Isotype Control

            Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            eBio440c

            Conjugate

            APC
            • Unconjugated
            • Brilliant UV 615
            • eFluor 450
            • eFluor 660
            • FITC
            • Functional Grade
            • PE 
            • PE-Cyanine7
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Request custom conjugation

            Excitation/Emission Max

            651/660 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2762688

            Product Specific Information

            Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

            It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

            Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

            Target Information

            Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: sialic acid binding Ig-like lectin H; Siglec; SIGLEC-like protein

            View more View less

            Gene Aliases: 6430529G09Rik; Siglec-H

            View more View less

            Entrez Gene ID: (Mouse) 233274

            View more View less

            Function(s)

            sialic acid binding cargo receptor activity immunoglobulin superfamily cell adhesion molecule

            Process(es)

            receptor-mediated endocytosis
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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