Invitrogen

CD16/CD32 Monoclonal Antibody (93), PE-Cyanine7, eBioscience™

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Tech Support

Antibody Testing Data (1)
Published Figures (7)

CD16/CD32 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

CD16/CD32 Antibody (25-0161-82) in Flow

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg of Anti-Mouse CD16/CD32 PE-Cyanine7 (filled histogram). Total cells were used for analysis.

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Product Details

25-0161-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 39 publications 39 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fruit fly, Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469598

Product Specific Information

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism.

Experimental hematology & oncology

Huang D,Han Y,Tang T,Yang L,Jiang P,Qian W,Zhang Z,Qian X,Zeng X,Qian P

25-0161-82 was used in Flow cytometry/Cell sorting to demonstrate that Dlk1 is essential for maintaining HSC homeostasis, which is realized by governing Notch signalling and restricting mitochondrial metabolic activity.

Wed Jan 18 00:00:00 EST 2023

Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice.

STAR protocols

Liu C,Lin X,Jin Y,Pan J

25-0161-82 was used in flow cytometry to describe practical procedures for sorting and analysis of leukemia stem cells (LSCs) to improve our understanding of chronic myelogenous leukemia (CML).

Fri Mar 17 00:00:00 EDT 2023

HDAC I/IIb selective inhibitor Purinostat Mesylate combined with GLS1 inhibition effectively eliminates CML stem cells.

Bioactive materials

Qiu Q,Yang L,Feng Y,Zhu Z,Li N,Zheng L,Sun Y,Pan C,Qiu H,Cui X,He W,Wang F,Yi Y,Tang M,Yang Z,Yang Y,Li Z,Chen L,Hu Y

25-0161-82 was used in Flow cytometry/Cell sorting to study the inhibition which eliminates CML stem cells.

Wed Mar 01 00:00:00 EST 2023

Nonredundancy of IL-1α and IL-1β is defined by distinct regulation of tissues orchestrating resistance versus tolerance to infection.

Science advances

Eislmayr K,Bestehorn A,Morelli L,Borroni M,Vande Walle L,Lamkanfi M,Kovarik P

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation.

Nature communications

Heil J,Olsavszky V,Busch K,Klapproth K,de la Torre C,Sticht C,Sandorski K,Hoffmann J,Schönhaber H,Zierow J,Winkler M,Schmid CD,Staniczek T,Daniels DE,Frayne J,Metzgeroth G,Nowak D,Schneider S,Neumaier M,Weyer V,Groden C,Gröne HJ,Richter K,Mogler C,Taketo MM,Schledzewski K,Géraud C,Goerdt S,Koch PS

25-0161-82 was used in Flow Cytometry to show that bone marrow sinusoidal endothelial cells actively control erythropoiesis.

Mon Nov 29 00:00:00 EST 2021

m⁶A Modification Prevents Formation of Endogenous Double-Stranded RNAs and Deleterious Innate Immune Responses during Hematopoietic Development.

Immunity

Gao Y,Vasic R,Song Y,Teng R,Liu C,Gbyli R,Biancon G,Nelakanti R,Lobben K,Kudo E,Liu W,Ardasheva A,Fu X,Wang X,Joshi P,Lee V,Dura B,Viero G,Iwasaki A,Fan R,Xiao A,Flavell RA,Li HB,Tebaldi T,Halene S

25-0161-82 was used in Flow Cytometry to investigate if N6-methyladenosine modification protects against endogenous double-stranded RNAs formation and a deleterious innate immune response during mammalian hematopoietic development.

Tue Jun 16 00:00:00 EDT 2020

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor.

Cancer cell

Di Genua C,Valletta S,Buono M,Stoilova B,Sweeney C,Rodriguez-Meira A,Grover A,Drissen R,Meng Y,Beveridge R,Aboukhalil Z,Karamitros D,Belderbos ME,Bystrykh L,Thongjuea S,Vyas P,Nerlov C

25-0161 was used in Flow cytometry/Cell sorting to show that combined biallelic Cebpa and Gata2 zinc finger-1 (ZnF1) mutations cooperatively induce bilineage AEL, and that the major leukemia-initiating cell (LIC) population has a neutrophil-monocyte progenitor (NMP) phenotype.

Mon May 11 00:00:00 EDT 2020

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor.

Cancer cell

Di Genua C,Valletta S,Buono M,Stoilova B,Sweeney C,Rodriguez-Meira A,Grover A,Drissen R,Meng Y,Beveridge R,Aboukhalil Z,Karamitros D,Belderbos ME,Bystrykh L,Thongjuea S,Vyas P,Nerlov C

Mon May 11 00:00:00 EDT 2020

RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia.

Cell stem cell

Shen C,Sheng Y,Zhu AC,Robinson S,Jiang X,Dong L,Chen H,Su R,Yin Z,Li W,Deng X,Chen Y,Hu YC,Weng H,Huang H,Prince E,Cogle CR,Sun M,Zhang B,Chen CW,Marcucci G,He C,Qian Z,Chen J

25-0161-82 was used in Flow Cytometry to reveal crucial functions of ALKBH5 in leukemogenesis and LSC/LIC self-renewal/maintenance and highlight the therapeutic potential of targeting the ALKBH5/m6A axis.

Thu Jul 02 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Site-1 protease ablation in the osterix-lineage in mice results in bone marrow neutrophilia and hematopoietic stem cell alterations.

Biology open

Patra D,Kim J,Zhang Q,Tycksen E,Sandell LJ

25-0161 was used in Flow cytometry/Cell sorting to show that S1P has a role in the lineage specification of hematopoietic stem cells and/or their progenitors for development of a normal hematopoietic niche.

Tue Jun 23 00:00:00 EDT 2020

Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter.

Cell reports

Eastman AE,Chen X,Hu X,Hartman AA,Pearlman Morales AM,Yang C,Lu J,Kueh HY,Guo S

25-0161 was used in Flow cytometry/Cell sorting to describe a genetically encoded fluorescent reporter that captures live-cell cycle speed using a single measurement.

Tue Jun 23 00:00:00 EDT 2020

Methylation of a CGATA element inhibits binding and regulation by GATA-1.

Nature communications

Yang L,Chen Z,Stout ES,Delerue F,Ittner LM,Wilkins MR,Quinlan KGR,Crossley M

25-0161 was used in Flow cytometry/Cell sorting to show that the erythroid transcription factor GATA-1 - that typically binds T/AGATA sites - can also recognise CGATA elements, but only if the CpG dinucleotide is unmethylated.

Fri May 22 00:00:00 EDT 2020

Resolving Fates and Single-Cell Transcriptomes of Hematopoietic Stem Cell Clones by PolyloxExpress Barcoding.

Cell stem cell

Pei W,Shang F,Wang X,Fanti AK,Greco A,Busch K,Klapproth K,Zhang Q,Quedenau C,Sauer S,Feyerabend TB,Höfer T,Rodewald HR

25-0161 was used in Flow cytometry/Cell sorting to develop the PolyloxExpress allele, enabling Cre-recombinase-dependent RNA barcoding in situ.

Thu Sep 03 00:00:00 EDT 2020

Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer.

Journal of hematology & oncology

Hwang WL,Lan HY,Cheng WC,Huang SC,Yang MH

25-0161 was used in Flow cytometry/Cell sorting to understand the interplay between heterogeneous tumour subpopulations and immunocytes, in modulating tumour immunity.

Fri Jan 25 00:00:00 EST 2019

Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host.

Nature

Meisel M,Hinterleitner R,Pacis A,Chen L,Earley ZM,Mayassi T,Pierre JF,Ernest JD,Galipeau HJ,Thuille N,Bouziat R,Buscarlet M,Ringus DL,Wang Y,Li Y,Dinh V,Kim SM,McDonald BD,Zurenski MA,Musch MW,Furtado GC,Lira SA,Baier G,Chang EB,Eren AM,Weber CR,Busque L,Godley LA,Verdú EF,Barreiro LB,Jabri B

25-0161 was used in Flow cytometry/Cell sorting to study the role played by bacterial translocation and increased interleukin-6 production in the development of pre-leukaemic myeloproliferation.

Tue May 01 00:00:00 EDT 2018

A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion.

Immunity

Xia P,Wang S,Ye B,Du Y,Li C,Xiong Z,Qu Y,Fan Z

25-0161 was used in Flow cytometry/Cell sorting to study circular RNA-mediated maintenance of hematopoeitic stem cell homeostasis.

Tue Apr 17 00:00:00 EDT 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Establishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points.

Epigenetics & chromatin

Heuston EF,Keller CA,Lichtenberg J,Giardine B,Anderson SM,Hardison RC,Bodine DM

25-0161 was used in Flow cytometry/Cell sorting to demonstrate that megakaryopoietic profiles are established early in haematopoiesis and are present in the majority of the haematopoietic progenitor population.

Mon May 28 00:00:00 EDT 2018

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

Direct Activation of BAX by BTSA1 Overcomes Apoptosis Resistance in Acute Myeloid Leukemia.

Cancer cell

Reyna DE,Garner TP,Lopez A,Kopp F,Choudhary GS,Sridharan A,Narayanagari SR,Mitchell K,Dong B,Bartholdy BA,Walensky LD,Verma A,Steidl U,Gavathiotis E

25-0161 was used in Flow cytometry/Cell sorting to identify BTSA1, a pharmacologically optimised BAX activator that binds with high affinity and specificity to the N-terminal activation site and induces conformational changes to BAX leading to BAX-mediated apoptosis.

Mon Oct 09 00:00:00 EDT 2017

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.

Cell stem cell

Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

25-0161 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone.

Thu Jun 01 00:00:00 EDT 2017

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

Core Circadian Clock Genes Regulate Leukemia Stem Cells in AML.

Cell

Puram RV,Kowalczyk MS,de Boer CG,Schneider RK,Miller PG,McConkey M,Tothova Z,Tejero H,Heckl D,Järås M,Chen MC,Li H,Tamayo A,Cowley GS,Rozenblatt-Rosen O,Al-Shahrour F,Regev A,Ebert BL

25-0161 was used in Flow cytometry/Cell sorting to identify essential transcription factors in a murine model of acute myeloid leukemia with genetically and phenotypically defined leukaemia stem cells.

Thu Apr 07 00:00:00 EDT 2016

Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells.

Cell

Yu VWC,Yusuf RZ,Oki T,Wu J,Saez B,Wang X,Cook C,Baryawno N,Ziller MJ,Lee E,Gu H,Meissner A,Lin CP,Kharchenko PV,Scadden DT

25016182 was used in flow cytometry to study epigenetic heterogeneity in hematopoietic stem cells.

Thu Nov 17 00:00:00 EST 2016

Hematopoietic Stem Cells Count and Remember Self-Renewal Divisions.

Cell

Bernitz JM,Kim HS,MacArthur B,Sieburg H,Moore K

25-0161 was used in Flow cytometry/Cell sorting to suggest that HSCs accumulate discrete memory stages over their divisional history and identify cellular memory in HSC aging.

Thu Nov 17 00:00:00 EST 2016

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Wed May 18 00:00:00 EDT 2016

SWEF Proteins Distinctly Control Maintenance and Differentiation of Hematopoietic Stem Cells.

PloS one

Ripich T,Chacón-Martínez CA,Fischer L,Pernis A,Kiessling N,Garbe AI,Jessberger R

25-0161 was used in Flow cytometry/Cell sorting to investigate the role of SWAP-70 and DEF6 in hematopoietic stem and precursor cells.

Mon Jul 31 00:00:00 EDT 2017

Geminin deletion increases the number of fetal hematopoietic stem cells by affecting the expression of key transcription factors.

Development (Cambridge, England)

Karamitros D,Patmanidi AL,Kotantaki P,Potocnik AJ,Bähr-Ivacevic T,Benes V,Lygerou Z,Kioussis D,Taraviras S

25-0161 was used in Flow cytometry/Cell sorting to investigate the role of geminin in hematopoiesis.

Thu Jan 01 00:00:00 EST 2015

Partial Loss of Rpl11 in Adult Mice Recapitulates Diamond-Blackfan Anemia and Promotes Lymphomagenesis.

Cell reports

Morgado-Palacin L,Varetti G,Llanos S,Gómez-López G,Martinez D,Serrano M

25-0161 was used in Flow cytometry/Cell sorting to conclude that Rpl11-deficient mice recapitulate DBA disorder, including cancer predisposition.

Tue Oct 27 00:00:00 EDT 2015

IFNγ induces monopoiesis and inhibits neutrophil development during inflammation.

Blood

de Bruin AM,Libregts SF,Valkhof M,Boon L,Touw IP,Nolte MA

25-0161 was used in Flow cytometry/Cell sorting to demonstrate that the pro-inflammatory cytokine IFNγ is involved in orchestrating inflammation-induced myelopoiesis.

Thu Feb 09 00:00:00 EST 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD16/CD32 monoclonal antibody (Product # 25-0161-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Chronic IFN-γ production in mice induces anemia by reducing erythrocyte life span and inhibiting erythropoiesis through an IRF-1/PU.1 axis.

Blood

Libregts SF,Gutiérrez L,de Bruin AM,Wensveen FM,Papadopoulos P,van Ijcken W,Ozgür Z,Philipsen S,Nolte MA

25-0161 was used in Flow cytometry/Cell sorting to reveal a molecular mechanism by which chronic exposure to IFN-γ induces expression of IRF-1 and ultimately induces anaemia.

Thu Sep 01 00:00:00 EDT 2011

Defective hematopoietic stem cell and lymphoid progenitor development in the Ts65Dn mouse model of Down syndrome: potential role of oxidative stress.

Antioxidants & redox signaling

Lorenzo LP,Chen H,Shatynski KE,Clark S,Yuan R,Harrison DE,Yarowsky PJ,Williams MS

25-0161 was used in Flow cytometry/Cell sorting to identify a link between oxidative stress and diminished IL-7Rα expression and function associated with defective haematologic development in a DS mouse model.

Sat Oct 15 00:00:00 EDT 2011

Eosinophil differentiation in the bone marrow is inhibited by T cell-derived IFN-gamma.

Blood

de Bruin AM,Buitenhuis M,van der Sluijs KF,van Gisbergen KP,Boon L,Nolte MA

25-0161 was used in Flow cytometry/Cell sorting to investigate whether and how T cells can affect myelopoiesis, showing that eosinophil differentiation in the bone marrow is inhibited by T cell-derived IFN-gamma.

Thu Oct 07 00:00:00 EDT 2010

Mcl1 haploinsufficiency protects mice from Myc-induced acute myeloid leukemia.

The Journal of clinical investigation

Xiang Z,Luo H,Payton JE,Cain J,Ley TJ,Opferman JT,Tomasson MH

25-0161 was used in Flow cytometry/Cell sorting to demonstrate a critical and dose-dependent role for Mcl1 in acute myelogenous leukaemia pathogenesis.

Tue Jun 01 00:00:00 EDT 2010

Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase.

Blood

Gupta R,Karpatkin S,Basch RS

25-0161 was used in Flow cytometry/Cell sorting to investigate the control of haematopoiesis by peroxide-sensitive regulatory mechanisms.

Wed Mar 01 00:00:00 EST 2006

Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase.

Blood

Gupta R,Karpatkin S,Basch RS

25-0161 was used in Flow cytometry/Cell sorting to investigate the control of haematopoiesis by peroxide-sensitive regulatory mechanisms.

Wed Mar 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1

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Gene Aliases: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

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Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin binding IgG receptor activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD16/CD32 Monoclonal Antibody (93), PE-Cyanine7, eBioscience™

CD16/CD32 Antibody (25-0161-82) in Flow

Product Details

25-0161-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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