Product References
Celecoxib induces adipogenic differentiation of hemangioma-derived mesenchymal stem cells through the PPAR-γ pathway in vitro and in vivo.
Experimental and therapeutic medicine
Wang Y,Kong L,Sun B,Cui J,Shen W
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed Jun 01 00:00:00 EDT 2022
Mitochondrial oxidative phosphorylation is dispensable for survival of CD34+ chronic myeloid leukemia stem and progenitor cells.
Cell death & disease
Yan JS,Yang MY,Zhang XH,Luo CH,Du CK,Jiang Y,Dong XJ,Wang ZM,Yang LX,Li YD,Xia L,Lu Y
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed Apr 20 00:00:00 EDT 2022
Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway.
Experimental and therapeutic medicine
Liu Y,Zhang X,Hu Y,Kang M,Wu Y,Wang Y,Deng C
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Nov 01 00:00:00 EDT 2022
Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling.
Scientific reports
Shin HS,Thakore A,Tada Y,Pedroza AJ,Ikeda G,Chen IY,Chan D,Jaatinen KJ,Yajima S,Pfrender EM,Kawamura M,Yang PC,Wu JC,Appel EA,Fischbein MP,Woo Y,Shudo Y
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Thu Oct 20 00:00:00 EDT 2022
Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.
Cell discovery
Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L
25-0349-42 was used in Flow cytometry/Cell sorting to demonstrate that interferon-mediated cell cycle control underlies the clinical efficacy of glucocorticoids.
Tue May 10 00:00:00 EDT 2022
LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.
International journal of medical sciences
Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Mon Aug 08 00:00:00 EDT 2022
Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.
The Journal of experimental medicine
Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Mon Feb 01 00:00:00 EST 2021
VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.
Bioengineered
Jiang W,Zhang J,Zhang X,Fan C,Huang J
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed Dec 01 00:00:00 EST 2021
Endothelial GABBR2 Regulates Post-ischemic Angiogenesis by Inhibiting the Glycolysis Pathway.
Frontiers in cardiovascular medicine
Zhang H,Zhou H,Yuan J,Nan Y,Liu J
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Aug 24 00:00:00 EDT 2021
TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.
Scientific reports
Chen C,Huang X,Zhu W,Ding C,Huang P,Li R
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Aug 31 00:00:00 EDT 2021
Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.
Stem cell research & therapy
Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Thu May 13 00:00:00 EDT 2021
Targeting the epichaperome as an effective precision medicine approach in a novel PML-SYK fusion acute myeloid leukemia.
NPJ precision oncology
Sugita M,Wilkes DC,Bareja R,Eng KW,Nataraj S,Jimenez-Flores RA,Yan L,De Leon JP,Croyle JA,Kaner J,Merugu S,Sharma S,MacDonald TY,Noorzad Z,Panchal P,Pancirer D,Cheng S,Xiang JZ,Olson L,Van Besien K,Rickman DS,Mathew S,Tam W,Rubin MA,Beltran H,Sboner A,Hassane DC,Chiosis G,Elemento O,Roboz GJ,Mosquera JM,Guzman ML
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed May 26 00:00:00 EDT 2021
Phenotypic and functional characterization of synovial fluid-derived fibroblast-like synoviocytes in rheumatoid arthritis.
Scientific reports
Køster D,Egedal JH,Lomholt S,Hvid M,Jakobsen MR,Müller-Ladner U,Eibel H,Deleuran B,Kragstrup TW,Neumann E,Nielsen MA
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Fri Nov 12 00:00:00 EST 2021
Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.
Stem cells international
Zhao AG,Shah K,Freitag J,Cromer B,Sumer H
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Oct 06 00:00:00 EDT 2020
Resveratrol rescues TNF‑α‑induced inhibition of osteogenesis in human periodontal ligament stem cells via the ERK1/2 pathway.
Molecular medicine reports
Yuan J,Wang X,Ma D,Gao H,Zheng D,Zhang J
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Fri May 01 00:00:00 EDT 2020
The downregulated membrane expression of CD18 in CD34+ cells defines a primitive population of human hematopoietic stem cells.
Stem cell research & therapy
Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E
25-0349 was used in Flow cytometry/Cell sorting to investigate if downregulated membrane expression of CD18 characterises a primitive population of human hematopoietic repopulating cells.
Tue Apr 28 00:00:00 EDT 2020
The downregulated membrane expression of CD18 in CD34+ cells defines a primitive population of human hematopoietic stem cells.
Stem cell research & therapy
Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E
25-0349 was used in Flow cytometry/Cell sorting to investigate if downregulated membrane expression of CD18 characterises a primitive population of human hematopoietic repopulating cells.
Tue Apr 28 00:00:00 EDT 2020
Genome-Wide Transcriptional Regulation of the Long Non-coding RNA Steroid Receptor RNA Activator in Human Erythroblasts.
Frontiers in genetics
Sawaengdee W,Cui K,Zhao K,Hongeng S,Fucharoen S,Wongtrakoongate P
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Mon Sep 28 00:00:00 EDT 2020
Chemerin enhances the adhesion and migration of human endothelial progenitor cells and increases lipid accumulation in mice with atherosclerosis.
Lipids in health and disease
Jia J,Yu F,Xiong Y,Wei W,Ma H,Nisi F,Song X,Yang L,Wang D,Yuan G,Zhou H
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Sun Sep 20 00:00:00 EDT 2020
Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.
Stem cell research & therapy
Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Fri Nov 27 00:00:00 EST 2020
Identification and characterization of the cellular subclones that contribute to the pathogenesis of mantle cell lymphoma.
Genes & diseases
Tang J,Zhang L,Zhou T,Sun Z,Kong L,Jing L,Xing H,Wu H,Liu Y,Zhou S,Li J,Chen M,Xu F,Tang J,Ma T,Hu M,Liu D,Guo J,Zhu X,Chen Y,Ye T,Wang J,Li X,Xing HR
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Sun Dec 01 00:00:00 EST 2019
Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.
Stem cells international
Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Thu May 13 00:00:00 EDT 2021
TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.
Molecular medicine reports
Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Oct 01 00:00:00 EDT 2019
Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis.
Nature communications
Kidoya H,Muramatsu F,Shimamura T,Jia W,Satoh T,Hayashi Y,Naito H,Kunisaki Y,Arai F,Seki M,Suzuki Y,Osawa T,Akira S,Takakura N
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed Mar 06 00:00:00 EST 2019
Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.
Cancers
Huang JY,Wang YY,Lo S,Tseng LM,Chen DR,Wu YC,Hou MF,Yuan SF
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Fri Dec 20 00:00:00 EST 2019
Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5.
BMC biotechnology
Lv Y,Xiao FJ,Wang Y,Zou XH,Wang H,Wang HY,Wang LS,Lu ZZ
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed Apr 24 00:00:00 EDT 2019
Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.
Cell reports
Mair B,Tomic J,Masud SN,Tonge P,Weiss A,Usaj M,Tong AHY,Kwan JJ,Brown KR,Titus E,Atkins M,Chan KSK,Munsie L,Habsid A,Han H,Kennedy M,Cohen B,Keller G,Moffat J
25-0349-42 was used in Flow Cytometry to establish parameters for genome-wide screens in hPSCs to facilitate the characterization of unappreciated genetic regulators of hPSC biology.
Tue Apr 09 00:00:00 EDT 2019
A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.
Developmental cell
Böiers C,Richardson SE,Laycock E,Zriwil A,Turati VA,Brown J,Wray JP,Wang D,James C,Herrero J,Sitnicka E,Karlsson S,Smith AJH,Jacobsen SEW,Enver T
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Mon Feb 05 00:00:00 EST 2018
Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.
Experimental and therapeutic medicine
Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Sat Dec 01 00:00:00 EST 2018
Improving hematopoietic recovery through modeling and modulation of the mesenchymal stromal cell secretome.
Stem cell research & therapy
Liu FD,Tam K,Pishesha N,Poon Z,Van Vliet KJ
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed Oct 24 00:00:00 EDT 2018
EDAG promotes the expansion and survival of human CD34+ cells.
PloS one
Zhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY
25-0349 was used in Flow cytometry/Cell sorting to provide novel insight into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells.
Tue Feb 06 00:00:00 EST 2018
Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.
Stem cells (Dayton, Ohio)
Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Sat Apr 01 00:00:00 EDT 2017
A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells.
Pulmonary circulation
Gaskill C,Majka SM
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed Mar 01 00:00:00 EST 2017
Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats.
Stem cell research & therapy
Yang H,Wu S,Feng R,Huang J,Liu L,Liu F,Chen Y
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Nov 21 00:00:00 EST 2017
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Stem cell research & therapy
Li J,Mao Q,He J,She H,Zhang Z,Yin C
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Thu Mar 09 00:00:00 EST 2017
Human Induced Pluripotent Stem Cell-Derived Macrophages Share Ontogeny with MYB-Independent Tissue-Resident Macrophages.
Stem cell reports
Buchrieser J,James W,Moore MD
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Feb 14 00:00:00 EST 2017
Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.
Stem cells translational medicine
Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Mon Aug 01 00:00:00 EDT 2016
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
Stem cells international
Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Jan 19 00:00:00 EST 2016
Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients.
PloS one
Muntión S,Ramos TL,Diez-Campelo M,Rosón B,Sánchez-Abarca LI,Misiewicz-Krzeminska I,Preciado S,Sarasquete ME,de Las Rivas J,González M,Sánchez-Guijo F,Del Cañizo MC
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Jul 12 00:00:00 EDT 2016
A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis.
Bone marrow research
Juneja SC,Viswanathan S,Ganguly M,Veillette C
25-0349 was used in Flow cytometry/Cell sorting to show that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis.
Fri Apr 08 00:00:00 EDT 2016
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.
NPJ Regenerative medicine
Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Wed Sep 30 00:00:00 EDT 2020
Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways.
Genome biology
Psaila B,Barkas N,Iskander D,Roy A,Anderson S,Ashley N,Caputo VS,Lichtenberg J,Loaiza S,Bodine DM,Karadimitris A,Mead AJ,Roberts I
25-0349 was used in Flow cytometry/Cell sorting to clarify the cellular hierarchy in human megakaryocyte/erythroid lineage commitment and highlight the importance of using a combination of single-cell approaches to dissect cellular heterogeneity.
Tue May 03 00:00:00 EDT 2016
Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.
American journal of physiology. Cell physiology
Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Mon Jun 15 00:00:00 EDT 2015
Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome.
Endocrinology
Oliva-Olivera W,Leiva Gea A,Lhamyani S,Coín-Aragüez L,Alcaide Torres J,Bernal-López MR,García-Luna PP,Morales Conde S,Fernández-Veledo S,El Bekay R,Tinahones FJ
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Tue Dec 01 00:00:00 EST 2015
SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.
Nature communications
Mian SA,Rouault-Pierre K,Smith AE,Seidl T,Pizzitola I,Kizilors A,Kulasekararaj AG,Bonnet D,Mufti GJ
25-0349 was used in Flow cytometry/Cell sorting to demonstrate that mutations to SF3B1 can arise from CD34(+)CD38(-)CD45RA(-)CD90(+)CD49f(+) haematopoietic stem cells and is an initiating event in myelodysplastic syndrome patients.
Tue Dec 08 00:00:00 EST 2015
Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.
Nature
Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S
25-0349 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.
Thu Jul 17 00:00:00 EDT 2014
Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.
Nature
Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S
25-0349 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.
Thu Jul 17 00:00:00 EDT 2014
JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34+ cells in vitro and in vivo.
Blood
Gallipoli P,Cook A,Rhodes S,Hopcroft L,Wheadon H,Whetton AD,Jørgensen HG,Bhatia R,Holyoake TL
Published figure using CD34 monoclonal antibody (Product # 25-0349-42) in Flow Cytometry
Thu Aug 28 00:00:00 EDT 2014
Developmental transcriptome analysis of human erythropoiesis.
Human molecular genetics
Shi L,Lin YH,Sierant MC,Zhu F,Cui S,Guan Y,Sartor MA,Tanabe O,Lim KC,Engel JD
25-0349 was used in Flow cytometry/Cell sorting to perform RNA-seq on CD34(+) cells after ex vivo differentiation from the earliest into the most mature erythroid cell stages.
Mon Sep 01 00:00:00 EDT 2014
Endothelial damage and vascular calcification in patients with chronic kidney disease.
American journal of physiology. Renal physiology
Soriano S,Carmona A,Triviño F,Rodriguez M,Alvarez-Benito M,Martín-Malo A,Alvarez-Lara MA,Ramírez R,Aljama P,Carracedo J
25-0349 was used in Flow cytometry/Cell sorting to investigate how EPCs, through OCN expression, may directly participate in the process of VC.
Mon Dec 01 00:00:00 EST 2014
Lysine-specific demethylase 1 is a therapeutic target for fetal hemoglobin induction.
Nature medicine
Shi L,Cui S,Engel JD,Tanabe O
25-0349 was used in Flow cytometry/Cell sorting to examine the effect of lysine-specific demethylase 1 inhibition on the expression of foetal γ-globin.
Fri Mar 01 00:00:00 EST 2013
CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.
Stem cells (Dayton, Ohio)
Gassenmaier M,Chen D,Buchner A,Henkel L,Schiemann M,Mack B,Schendel DJ,Zimmermann W,Pohla H
25-0349 was used in Flow cytometry/Cell sorting to study the expression of CXC chemokine receptor 4 in a subpopulation of tumour-initiating cells in renal cell carcinoma cell lines and study its role in their maintenance.
Thu Aug 01 00:00:00 EDT 2013
Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
PloS one
Rosu-Myles M,She YM,Fair J,Muradia G,Mehic J,Menendez P,Prasad SS,Cyr TD
25-0349 was used in Flow cytometry/Cell sorting to provide evidence of heterogeneity among bone marrow stromal cells and identify potential candidate proteins for quantifying MSCs in vitro.
Thu Dec 13 00:00:00 EST 2012