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Invitrogen
CD28 Monoclonal Antibody (CD28.2), Brilliant Ultra Violet™ 737, eBioscience™
FIGURE: 1 / 1
CD28 Antibody (367-0289-42) in Flow
Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet 737 (Product # 367-4714-81) (left) or CD28 Monoclonal Antibody, Brilliant Ultra Violet 737 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
367-0289-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CD28.2
Conjugate
Excitation/Emission Max
350/740 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937114
Product Specific Information
Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.
Applications Reported: This CD28.2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This CD28.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.
Our internal testing suggests that Brilliant Ultra Violet™ 737 (BUV737) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour.
Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44
Gene Aliases: CD28; Tp44
UniProt ID: (Human) P10747
Entrez Gene ID: (Human) 940
positive regulation of inflammatory response to antigenic stimulus
humoral immune response
cell surface receptor signaling pathway
positive regulation of gene expression
negative regulation of gene expression
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
T cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-4 production
cytokine biosynthetic process
positive regulation of T cell proliferation
negative thymic T cell selection
regulatory T cell differentiation
positive regulation of viral genome replication
positive regulation of interleukin-2 biosynthetic process
regulation of regulatory T cell differentiation
positive regulation of translation
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
positive regulation of alpha-beta T cell proliferation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of isotype switching to IgG isotypes
regulation of defense response to virus by virus
T cell receptor signaling pathway
positive regulation of protein kinase B signaling
apoptotic signaling pathway
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