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Invitrogen
CD102 (ICAM-2) Monoclonal Antibody (3C4 (mIC2/4)), eFluor™ 450, eBioscience™
-Flow-20170216130641.jpg?time=20240507 "CD102 (ICAM-2) Antibody in Flow Cytometry (Flow)")
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CD102 (ICAM-2) Antibody (48-1021-82) in Flow
Staining of C57BL/6 splenocytes with 0.5 µg of Rat IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4321-82) (blue histogram) or 0.5 µg of Anti-Mouse CD102 (ICAM-2) eFluor® 450 (purple histogram). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20240507)
-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD102 (ICAM-2) Antibody (48-1021-82) in Flow")
Product Details
48-1021-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
3C4 (mIC2/4)
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1548749
Product Specific Information
Description: The monoclonal antibody 3C4 (mIC2/4) recognizes mouse CD102, also known as intercellular adhesion molecule-2 (ICAM-2). CD102, a member of the Immunoglobulin superfamily of receptors with 2 extracellular Ig domains, binds LFA (CD11a/CD18) and Mac-1 (CD11b/CD18), thereby mediating cellular interactions to lymphocytes. CD102 plays a role in NK cytotoxicity and migration, and lymphocyte recirculation. Expression of CD102 is found on most lymphocytes, monocytes and platelets.
The 3C4 (mIC2/4) antibody blocks interaction of lymphocytes with antigen presenting cells.
Applications Reported: This 3C4 (mIC2/4) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3C4 (mIC2/4) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ICAM-2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM-2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. Diseases associated with ICAM2 include Colon Carcinoma In Situ.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD102; ICAM-2; Intercellular adhesion molecule 2; Lymphocyte function-associated AG-1 counter-receptor; RP23-418O11.10-001
Gene Aliases: CD102; Icam-2; Icam2; Ly-60
UniProt ID: (Mouse) P35330
Entrez Gene ID: (Mouse) 15896
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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