Product References
ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.
Nature communications
Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Fri May 19 00:00:00 EDT 2023
Small-molecule PTPN2 Inhibitors Sensitize Resistant Melanoma to Anti-PD-1 Immunotherapy.
Cancer research communications
Zhu Z,Tang R,Huff S,Kummetha IR,Wang L,Li N,Rana TM
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Sun Jan 01 00:00:00 EST 2023
The altering cellular components and function in tumor microenvironment during remissive and relapsed stages of anti-CD19 CAR T-cell treated lymphoma mice.
Frontiers in immunology
Zhao K,Ren C,Tang D,Zhao L,Chen X,Wang Y,Xu K
48-8898-82 was used in Flow cytometry/Cell sorting to find that anti-CD19 CAR T-cell treatment attenuated the symptoms of lymphoma and significantly prolonged mice survival through eradicating systemic CD19+ cells.
Mon Feb 13 00:00:00 EST 2023
Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.
The Journal of clinical investigation
Ren J,Li N,Pei S,Lian Y,Li L,Peng Y,Liu Q,Guo J,Wang X,Han Y,Zhang G,Wang H,Li Y,Jiang J,Li Q,Tan M,Peng J,Hu G,Xiao Y,Li X,Lin M,Qin J
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Fri Apr 15 00:00:00 EDT 2022
Targeting Fc Receptor-Mediated Effects and the "Don't Eat Me" Signal with an Oncolytic Virus Expressing an Anti-CD47 Antibody to Treat Metastatic Ovarian Cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research
Tian L,Xu B,Teng KY,Song M,Zhu Z,Chen Y,Wang J,Zhang J,Feng M,Kaur B,Rodriguez L,Caligiuri MA,Yu J
48-8898-82 was used in Flow cytometry/Cell sorting to improve therapeutic efficacy while reducing toxicities for ovarian cancer.
Sat Jan 01 00:00:00 EST 2022
Wnt5A Signaling Blocks Progression of Experimental Visceral Leishmaniasis.
Frontiers in immunology
Maity S,Chakraborty A,Mahata SK,Roy S,Das AK,Sen M
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Mar 22 00:00:00 EDT 2022
Intranasal Delivery of Recombinant S100A8 Protein Delays Lung Cancer Growth by Remodeling the Lung Immune Microenvironment.
Frontiers in immunology
Wong SW,McCarroll J,Hsu K,Geczy CL,Tedla N
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Mon Jun 06 00:00:00 EDT 2022
eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.
Journal for immunotherapy of cancer
Chen X,Wang K,Jiang S,Sun H,Che X,Zhang M,He J,Wen Y,Liao M,Li X,Zhou X,Song J,Ren X,Yi W,Yang J,Chen X,Yin M,Cheng Y
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Mar 01 00:00:00 EST 2022
eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.
Journal for immunotherapy of cancer
Chen X,Wang K,Jiang S,Sun H,Che X,Zhang M,He J,Wen Y,Liao M,Li X,Zhou X,Song J,Ren X,Yi W,Yang J,Chen X,Yin M,Cheng Y
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Mar 01 00:00:00 EST 2022
RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer.
The Journal of clinical investigation
Deng P,Wang Z,Chen J,Liu S,Yao X,Liu S,Liu L,Yu Z,Huang Y,Xiong Z,Xiao R,Gao J,Liang W,Chen J,Liu H,Hong JH,Chan JY,Guan P,Chen J,Wang Y,Yin J,Li J,Zheng M,Zhang C,Zhou P,Kang T,Teh BT,Yu Q,Zuo Z,Jiang Q,Liu J,Xiong Y,Xia X,Tan J
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Nov 15 00:00:00 EST 2022
PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.
Nature communications
Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X
48-8898-82 was used in Flow Cytometry to demonstrate that the PRDM1-USP22-SPI1 axis regulates PD-L1 levels, resulting in infiltrated CD8+ T cell exhaustion.
Mon Dec 12 00:00:00 EST 2022
PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.
Nature communications
Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X
48-8898-82 was used in Flow Cytometry to demonstrate that the PRDM1-USP22-SPI1 axis regulates PD-L1 levels, resulting in infiltrated CD8+ T cell exhaustion.
Mon Dec 12 00:00:00 EST 2022
Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.
Cell reports
Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Nov 01 00:00:00 EDT 2022
Specific targeting of glioblastoma with an oncolytic virus expressing a cetuximab-CCL5 fusion protein via innate and adaptive immunity.
Nature cancer
Tian L,Xu B,Chen Y,Li Z,Wang J,Zhang J,Ma R,Cao S,Hu W,Chiocca EA,Kaur B,Caligiuri MA,Yu J
48-8898-82 was used in Flow cytometry/Cell sorting to demonstrate that OV-Cmab-CCL5 offers a promising approach to improve OV therapy for solid tumors.
Tue Nov 01 00:00:00 EDT 2022
CD36-mediated ferroptosis dampens intratumoral CD8+ T cell effector function and impairs their antitumor ability.
Cell metabolism
Ma X,Xiao L,Liu L,Ye L,Su P,Bi E,Wang Q,Yang M,Qian J,Yi Q
48-8898-82 was used in Flow cytometry/Cell sorting to reveal a new mechanism of CD36 regulating the function of CD8+ effector T cells and therapeutic potential of targeting CD36 or inhibiting ferroptosis to restore T cell function.
Tue May 04 00:00:00 EDT 2021
Blockade of 6-phosphogluconate dehydrogenase generates CD8+ effector T cells with enhanced anti-tumor function.
Cell reports
Daneshmandi S,Cassel T,Lin P,Higashi RM,Wulf GM,Boussiotis VA,Fan TW,Seth P
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Mar 09 00:00:00 EST 2021
Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy.
Cell reports
Dodagatta-Marri E,Ma HY,Liang B,Li J,Meyer DS,Chen SY,Sun KH,Ren X,Zivak B,Rosenblum MD,Headley MB,Pinzas L,Reed NI,Del Cid JS,Hann BC,Yang S,Giddabasappa A,Noorbehesht K,Yang B,Dal Porto J,Tsukui T,Niessen K,Atakilit A,Akhurst RJ,Sheppard D
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Jul 06 00:00:00 EDT 2021
Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration.
Cell communication and signaling : CCS
Li J,Wang S,Wang N,Zheng Y,Yang B,Wang X,Zhang J,Pan B,Wang Z
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Mon Aug 30 00:00:00 EDT 2021
Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages.
International journal of nanomedicine
Luo ZW,Xia K,Liu YW,Liu JH,Rao SS,Hu XK,Chen CY,Xu R,Wang ZX,Xie H
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Jun 01 00:00:00 EDT 2021
Non-redundant activity of GSK-3α and GSK-3β in T cell-mediated tumor rejection.
iScience
Steele L,Mannion AJ,Shaw G,Maclennan KA,Cook GP,Rudd CE,Taylor A
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Fri Jun 25 00:00:00 EDT 2021
Circadian Clock Disruption Suppresses PDL1+ Intraepithelial B Cells in Experimental Colitis and Colitis-Associated Colorectal Cancer.
Cellular and molecular gastroenterology and hepatology
Liu JL,Wang CY,Cheng TY,Rixiati Y,Ji C,Deng M,Yao S,Yuan LH,Zhao YY,Shen T,Li JM
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Mon Jan 24 00:00:00 EST 2022
A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice.
Med (New York, N.Y.)
Silva-Cayetano A,Foster WS,Innocentin S,Belij-Rammerstorfer S,Spencer AJ,Burton OT,Fra-Bidó S,Le Lee J,Thakur N,Conceicao C,Wright D,Barrett J,Evans-Bailey N,Noble C,Bailey D,Liston A,Gilbert SC,Lambe T,Linterman MA
48-8898-82 was used in Flow Cytometry to show that ChAdOx1 nCoV-19 induces both cellular and humoral immunity in adult and aged mice and suggest a prime-boost strategy is a rational approach to enhance immunogenicity in older persons.
Fri Mar 12 00:00:00 EST 2021
Knockout of immunotherapy prognostic marker genes eliminates the effect of the anti-PD-1 treatment.
NPJ precision oncology
Yang N,Ji F,Cheng L,Lu J,Sun X,Lin X,Lan X
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Fri May 07 00:00:00 EDT 2021
Knockout of immunotherapy prognostic marker genes eliminates the effect of the anti-PD-1 treatment.
NPJ precision oncology
Yang N,Ji F,Cheng L,Lu J,Sun X,Lin X,Lan X
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Fri May 07 00:00:00 EDT 2021
Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity.
Journal for immunotherapy of cancer
Wang Z,Xu F,Hu J,Zhang H,Cui L,Lu W,He W,Wang X,Li M,Zhang H,Xiong W,Xie C,Liu Y,Zhou P,Liu J,Huang P,Qin XF,Xia X
48-8898-82 was used in Flow Cytometry to show that clotrimazole could trigger DC activation via the lactate-lysosome axis to promote antigen cross-presentation and could be used as a potential combination therapy approach to improving the therapeutic efficacy of anti-PD1 immunotherapy.
Sat May 01 00:00:00 EDT 2021
Identification of HLA-A2-Restricted Mutant Epitopes from Neoantigens of Esophageal Squamous Cell Carcinoma.
Vaccines
Wang Z,Ran L,Chen C,Shi R,Dong Y,Li Y,Zhou X,Qi Y,Zhu P,Gao Y,Wu Y
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Protein Assays and Analysis
Fri Oct 01 00:00:00 EDT 2021
Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2.
BioMed research international
Zhao K,Tian Y,Wang J,Chen C,Pan B,Yan Z,Zhu S,Xu K
48-8898 was used in Flow cytometry/Cell sorting to conclude that administration of Fluvastatin to donor mice showed protective effects against recipient aGVHD when compared to untreated mice due to the retention of effector T cells in lymphoid organs accompanying with reduction of nonlymphatic infiltration and related inflammatory cytokines.
Fri Sep 10 00:00:00 EDT 2021
Assessment of the WAP-Myc mouse mammary tumor model for spontaneous metastasis.
Scientific reports
Utz B,Turpin R,Lampe J,Pouwels J,Klefström J
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Fri Oct 30 00:00:00 EDT 2020
Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.
Cell reports
Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Jan 14 00:00:00 EST 2020
Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.
Biomedicines
Ebelt ND,Zuniga E,Marzagalli M,Zamloot V,Blazar BR,Salgia R,Manuel ER
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Wed Dec 16 00:00:00 EST 2020
Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.
Nature communications
Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L
48-8898 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.
Mon Dec 07 00:00:00 EST 2020
Memory CD73+IgM+ B cells protect against Plasmodium yoelii infection and express Granzyme B.
PloS one
Parra M,Weitner M,Yang A,Akue A,Liu X,Schmidt T,Allman WR,Akkoyunlu M,Derrick SC
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Oct 27 00:00:00 EDT 2020
cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity.
The Journal of clinical investigation
Wang Z,Chen J,Hu J,Zhang H,Xu F,He W,Wang X,Li M,Lu W,Zeng G,Zhou P,Huang P,Chen S,Li W,Xia LP,Xia X
48-8898 was used in Flow cytometry/Cell sorting to show that teniposide could trigger tumor immunogenicity and enable a potential chemoimmunotherapeutic approach to potentiating the therapeutic efficacy of anti-PD1 immunotherapy.
Tue Aug 13 00:00:00 EDT 2019
Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.
Nature communications
Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Thu Nov 28 00:00:00 EST 2019
A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence.
Immunity
Mamedov MR,Scholzen A,Nair RV,Cumnock K,Kenkel JA,Oliveira JHM,Trujillo DL,Saligrama N,Zhang Y,Rubelt F,Schneider DS,Chien YH,Sauerwein RW,Davis MM
48-8898-82 was used in Flow Cytometry to point to an M-CSF-producing γδ T cell subset that fulfills a specialized protective role in the later stage of malaria infection when αβ T cells have declined.
Tue Feb 20 00:00:00 EST 2018
MAIT cells contribute to protection against lethal influenza infection in vivo.
Nature communications
van Wilgenburg B,Loh L,Chen Z,Pediongco TJ,Wang H,Shi M,Zhao Z,Koutsakos M,Nüssing S,Sant S,Wang Z,D'Souza C,Jia X,Almeida CF,Kostenko L,Eckle SBG,Meehan BS,Kallies A,Godfrey DI,Reading PC,Corbett AJ,McCluskey J,Klenerman P,Kedzierska K,Hinks TSC
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Fri Nov 09 00:00:00 EST 2018
Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.
Nature communications
Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Mon Nov 19 00:00:00 EST 2018
Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.
Nature communications
Kim DH,Park HJ,Lim S,Koo JH,Lee HG,Choi JO,Oh JH,Ha SJ,Kang MJ,Lee CM,Lee CG,Elias JA,Choi JM
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Mon Feb 05 00:00:00 EST 2018
The immunocytokine L19-IL2: An interplay between radiotherapy and long-lasting systemic anti-tumour immune responses.
Oncoimmunology
Rekers NH,Olivo Pimentel V,Yaromina A,Lieuwes NG,Biemans R,Zegers CML,Germeraad WTV,Van Limbergen EJ,Neri D,Dubois LJ,Lambin P
48-8898 was used in Flow cytometry/Cell sorting to show that radiotherapy and L19-IL2 can activate a curative abscopal effect, with a long-lasting immunological memory.
Tue Jan 12 00:00:00 EST 2021
Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.
Science translational medicine
Allen E,Jabouille A,Rivera LB,Lodewijckx I,Missiaen R,Steri V,Feyen K,Tawney J,Hanahan D,Michael IP,Bergers G
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Wed Apr 12 00:00:00 EDT 2017
Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.
The Journal of experimental medicine
Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Mon Jun 05 00:00:00 EDT 2017
1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8+ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model.
Frontiers in immunology
Dai K,Huang L,Huang YB,Chen ZB,Yang LH,Jiang YA
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Wed Nov 20 00:00:00 EST 2019
Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.
Nature communications
Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Fri Nov 17 00:00:00 EST 2017
The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.
Oncoimmunology
Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Jan 12 00:00:00 EST 2021
Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology.
Journal of virology
Teo TH,Her Z,Tan JJ,Lum FM,Lee WW,Chan YH,Ong RY,Kam YW,Leparc-Goffart I,Gallian P,Rénia L,de Lamballerie X,Ng LF
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Immunohistochemistry
Sat Aug 01 00:00:00 EDT 2015
Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology.
Journal of virology
Teo TH,Her Z,Tan JJ,Lum FM,Lee WW,Chan YH,Ong RY,Kam YW,Leparc-Goffart I,Gallian P,Rénia L,de Lamballerie X,Ng LF
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Immunohistochemistry
Sat Aug 01 00:00:00 EDT 2015
Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.
Nature communications
Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Tue Oct 28 00:00:00 EDT 2014
Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.
PloS one
Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z
Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry
Mon Jul 06 00:00:00 EDT 2015
