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Invitrogen
5-Methylcytidine Monoclonal Antibody (33D3)
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Application
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5-Methylcytidine Antibody (MA1-80331) in ChIP
Matrix MeDIP in human brain was performed using a monoclonal antibody targeting specifically 5mC methylated DNA (Product # MA1-80331). Differences in at ActB transcription start site (TSS) and H19 imprinting control region (ICR) for human brain are graphed by fraction of input. For all brain samples, P = <0.003 for ActB vs H19, and for H19 5mC vs IgG (two-tailed, two-sample t-Test). Data courtesy of Dr. Karol Bomsztyk laboratory.
Product Details
MA1-80331
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
33D3
Immunogen
Proprietary Immunogen.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_926789
Product Specific Information
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti 5-methylcytidine antibody, clone 33D3 recognizes 5-methylcytidine, a modified base found in the DNA of plants and vertebrates.
Target Information
Modified base 5-methyl cytidine (5-MeCyd) is found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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