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CCL14 Antibody (500-P106-1MG) in WB
Western Blot: To detect Human HCC-1 (CCL14) by Western Blot analysis CCL14 Polyclonal Antibody (Product # 500-P106-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human HCC-1 (CCL14) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P106-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human HCC-1 (CCL14) (72 a.a.)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929217
Product Specific Information
AA Sequence of recombinant protein: TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human HCC-1 (CCL14). Anti-Human HCC-1 (CCL14)-specific antibody was purified by affinity chromatography employing an immobilized Human HCC-1 (CCL14) matrix.
Sandwich ELISA: To detect Human HCC-1 (CCL14) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human HCC-1 (CCL14) (500-P106Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human HCC-1 (CCL14).
Western Blot: To detect Human HCC-1 (CCL14) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human HCC-1 (CCL14) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This gene, CCL14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts. Bicistronic transcripts include the upstream cytokine gene CCL15. In addition, CCL14 undergoes alternative splicing in the bicistronic transcripts; it is unknown if its monocistronic transcript is also subject to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 14; chemokine (C-C motif) ligand 14; Chemokine CC-1/CC-3; chemokine CC-3; H HCC-1(1-74); HCC-1(1-74); HCC-1/HCC-3; hemofiltrate CC chemokine 1; NCC-2; new CC chemokine 2; small inducible cytokine subfamily A (Cys-Cys), member 14; Small-inducible cytokine A14
Gene Aliases: CC-1; CC-3; CCL14; CKB1; HCC-1; HCC-1(1-74); HCC-1/HCC-3; HCC-3; MCIF; NCC-2; NCC2; SCYA14; SCYL2; SY14
UniProt ID: (Human) Q16627
Entrez Gene ID: (Human) 6358
monocyte chemotaxis
cellular calcium ion homeostasis
inflammatory response
G-protein coupled receptor signaling pathway
positive regulation of cell proliferation
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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