Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
CXCL6 Antibody (500-P120BT-1MG) in WB
Western Blot: To detect hGCP-2 by Western Blot analysis CXCL6 Polyclonal Antibody, Biotin (Product # 500-P120BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P120BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human GCP-2 (CXCL6)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929318
Product Specific Information
AA Sequence of recombinant protein: VLTELRCTCL RVTLRVNPKT IGKLQVFPAG PQCSKVEVVA SLKNGKQVCL DPEAPFLKKV IQKILDSGNK KN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GCP-2 (CXCL6). Anti-Human GCP-2 (CXCL6)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human GCP-2 (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GCP-2 (CXCL6) (500-P120) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GCP-2 (CXCL6).
Western Blot: To detect hGCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P120BT-1MG will be provided as 2 x 500 µg
Target Information
CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support