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Invitrogen
VEGF-165 Polyclonal Antibody, Biotin, PeproTech®
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VEGF-165 Antibody (500-P131BT-1MG) in WB
Western Blot: To detect mVEGF by Western Blot analysis VEGF-165 Polyclonal Antibody, Biotin (Product # 500-P131BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P131BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 39.0 kDa Recombinant Murine VEGF 165
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929391
Product Specific Information
AA Sequence of recombinant protein: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine VEGF 165. Anti-Murine VEGF 165-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine VEGF 165 (500-P131) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine VEGF 165.
Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P131BT-1MG will be provided as 2 x 500 µg
Target Information
The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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