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TWEAK Antibody (500-P137GBT-1MG) in WB
Western Blot: To detect hTWEAK by Western Blot analysis TWEAK Polyclonal Antibody, Biotin (Product # 500-P137GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTWEAK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P137GBT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 17.0 kDa Recombinant Human TWEAK
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929429
Product Specific Information
AA Sequence of recombinant protein: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human TWEAK. Anti-Human TWEAK-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human TWEAK by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TWEAK (500-P137G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TWEAK.
Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTWEAK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P137GBT-1MG will be provided as 2 x 500 µg
Target Information
TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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