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CNTF Antibody (500-P140BT-1MG) in WB
Western Blot: To detect hCNTF by Western Blot analysis CNTF Polyclonal Antibody, Biotin (Product # 500-P140BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hCNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P140BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human CNTF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929450
Product Specific Information
AA Sequence of recombinant protein: AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTQSYVKH QGLNKNINLD SADGMPVAST DQWSQLTQAQ RLQQNLQAYR TFHVLLARLL QDQQVHFTPT QGDFHQAIHT LLLQVAAFAY QIQQLMILLQ YKIPRNQADG MPINVGDGGL FQKKLWGLKV LQQLSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CNTF. Anti-Human CNTF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human CNTF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human CNTF (500-P140) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CNTF.
Western Blot: To detect hCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hCNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P140BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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