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Eotaxin 2 Antibody (500-P175GBT-1MG) in ELISA
Sandwich ELISA: To detect mEotaxin-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Eotaxin 2 Polyclonal Antibody, Biotin (Product # 500-P175GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Eotaxin 2 Polyclonal Antibody (Product # 500-P175G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mEotaxin-2.
Product Details
500-P175GBT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine Eotaxin-2 (CCL24)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929645
Product Specific Information
AA Sequence of recombinant protein: VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine Eotaxin-2 (CCL24). Anti-Murine Eotaxin-2 (CCL24)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mEotaxin-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Eotaxin-2 (500-P175G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mEotaxin-2.
Western Blot: To detect mEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mEotaxin-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P175GBT-1MG will be provided as 2 x 500 µg
Target Information
Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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