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IL-19 Antibody (500-P189BT-1MG) in WB
Western Blot: To detect Human IL-19 by Western Blot analysis IL-19 Polyclonal Antibody, Biotin (Product # 500-P189BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-19 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P189BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-19
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929735
Product Specific Information
AA Sequence of recombinant protein: MLRRCLISTD MHHIEESFQE IKRAIQAKDT FPNVTILSTL ETLQIIKPLD VCCVTKNLLA FYVDRVFKDH QEPNPKILRK ISSIANSFLY MQKTLRQCQE QRQCHCRQEA TNATRVIHDN YDQLEVHAAA IKSLGELDVF LAWINKNHEV MSSA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-19. Anti-Human IL-19-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIL-19 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-19 (500-P189) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-19.
Western Blot: To detect Human IL-19 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-19 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P189BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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