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IL-10 Antibody (500-P20BT-1MG) in WB
Western Blot: To detect hIL-10 by Western Blot analysis IL-10 Polyclonal Antibody, Biotin (Product # 500-P20BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P20BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-10
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929822
Product Specific Information
AA Sequence of recombinant protein: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIL-10 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-10 (500-P20) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-10.
Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P20BT-1MG will be provided as 2 x 500 µg
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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