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IGFBP-1 Antibody (500-P228-1MG) in WB
Western Blot: To detect Human IGF-BP1 by Western Blot analysis IGFBP-1 Polyclonal Antibody (Product # 500-P228-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-BP1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P228-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IGF-BP1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929919
Product Specific Information
AA Sequence of recombinant protein: MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN.
Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human IGF-BP1. Anti-Human IGF-BP1-specific antibody was purified by affinity chromatography employing an immobilized Human IGF-BP1 matrix.
Sandwich ELISA: To detect Human IGF-BP1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IGF-BP1 (500-P228Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-BP1.
Western Blot: To detect Human IGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-BP1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; Binding protein 25; Binding protein 26; Binding protein 28; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP 1; hIGFBP1; IBP 1; IBP-1; IGF BP25; IGF-binding protein 1; IGFBP; IGFBP 1; IGFBP-1; Insulin like growth factor binding protein; insulin-like growth factor binding protein 1; Insulin-like growth factor-binding protein 1; Placental protein 12; PP 12; PP12
Gene Aliases: AFBP; hIGFBP-1; IBP1; IGF-BP25; IGFBP1; PP12
UniProt ID: (Human) P08833
Entrez Gene ID: (Human) 3484
signal transduction
insulin receptor signaling pathway
positive regulation of cell growth
PERK-mediated unfolded protein response
tissue regeneration
regulation of insulin-like growth factor receptor signaling pathway
cellular protein metabolic process
negative regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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