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IL-3 Antibody (500-P23BT-1MG) in WB
Western Blot: To detect hIL-3 by Western Blot analysis IL-3 Polyclonal Antibody, Biotin (Product # 500-P23BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P23BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-3
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929990
Product Specific Information
AA Sequence of recombinant protein: APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-3. Anti-Human IL-3-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIL-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-3 (500-P23) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-3.
Western Blot: To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P23BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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