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MIA Antibody (500-P243-1MG) in WB
Western Blot: To detect Human MIA by Western Blot analysis MIA Polyclonal Antibody (Product # 500-P243-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P243-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MIA
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930014
Product Specific Information
AA Sequence of recombinant protein: MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIA. Anti-Human MIA-specific antibody was purified by affinity chromatography employing an immobilized Human MIA matrix.
Sandwich ELISA: To detect Human MIA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIA (500-P243Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIA.
Western Blot: To detect Human MIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Tumorigenesis is a process that is mediated by a network of membrane, cytosolic and nuclear associated factors, which regulate proliferation and cell-matrix interaction through signaling cascades. The phenotype of malignant melanomas in vivo depends on the global expression of stimulatory or inhibitory factors generated in both the tumors cells and their environment. One example, Melanoma inhibitory activity (cartilage-derived retinoic acid-sensitive protein (CD-RAP), MIA) is a Src homology 3 (SH3)-like domain containing protein that is secreted from chondrocytes and malignant melanoma cells. MIA is translated as a 131-amino acid pro-form and processed into a mature 107-amino acid protein after cleavage of a secretion signal. MIA is expressed during chondrogenesis and in mature chondrocytes, suggesting that MIA is necessary for normal cartilage cell phenotype. MIA mRNA is present in carcinomas of the colon, ovary, kidney, and head/neck, and may represent a marker to monitor melanomic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Melanoma inhibitory activity protein; Melanoma-derived growth regulatory protein
Gene Aliases: CD-RAP; MIA
UniProt ID: (Human) Q16674
Entrez Gene ID: (Human) 8190
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