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IL-7 Antibody (500-P27BT-1MG) in WB
Western Blot: To detect Human IL-7 by Western Blot analysis IL-7 Polyclonal Antibody, Biotin (Product # 500-P27BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant hIL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P27BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-7
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930224
Product Specific Information
AA Sequence of recombinant protein: MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-7. Anti-Human IL-7-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human IL-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-7 (500-P27) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-7.
Western Blot: To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant hIL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P27BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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