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CCL28 Antibody (500-P297BT-1MG) in WB
Western Blot: To detect hMEC by Western Blot analysis CCL28 Polyclonal Antibody, Biotin (Product # 500-P297BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P297BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MEC (CCL28)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930287
Product Specific Information
AA Sequence of recombinant protein: SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MEC (CCL28). Anti-Human MEC (CCL28)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hMEC by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MEC (500-P297) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMEC.
Western Blot: To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P297BT-1MG will be provided as 2 x 500 µg
Target Information
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor, and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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