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VAP-1 Antibody (500-P326-1MG) in WB
Western Blot: To detect Human VAP-1 by Western Blot analysis VAP-1 Polyclonal Antibody (Product # 500-P326-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human VAP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P326-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human VAP-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930429
Product Specific Information
AA Sequence of recombinant protein: GRGGDGGEPS QLPHCPSVSP SAQPWTHPGQ SQLFADLSRE ELTAVMRFLT QRLGPGLVDA AQARPSDNCV FSVELQLPPK AAALAHLDRG SPPPAREALA IVFFGRQPQP NVSELVVGPL PHPSYMRDVT VERHGGPLPY HRRPVLFQEY LDIDQMIFNR ELPQASGLLH HCCFYKHRGR NLVTMTTAPR GLQSGDRATW FGLYYNISGA GFFLHHVGLE LLVNHKALDP ARWTIQKVFY QGRYYDSLAQ LEAQFEAGLV NVVLIPDNGT GGSWSLKSPV PPGPAPPLQF YPQGPRFSVQ GSRVASSLWT FSFGLGAFSG PRIFDVRFQG ERLVYEISLQ EALAIYGGNS PAAMTTRYVD GGFGMGKYTT PLTRGVDCPY LATYVDWHFL LESQAPKTIR DAFCVFEQNQ GLPLRRHHSD LYSHYFGGLA ETVLVVRSMS TLLNYDYVWD TVFHPSGAIE IRFYATGYIS SAFLFGATGK YGNQVSEHTL GTVHTHSAHF KVDLDVAGLE NWVWAEDMVF VPMAVPWSPE HQLQRLQVTR KLLEMEEQAA FLVGSATPRY LYLASNHSNK WGHPRGYRIQ MLSFAGEPLP QNSSMARGFS WERYQLAVTQ RKEEEPSSSS VFNQNDPWAP TVDFSDFINN ETIAGKDLVA WVTAGFLHIP HAEDIPNTVT VGNGVGFFLR PYNFFDEDPS FYSADSIYFR GDQDAGACEV NPLACLPQAA ACAPDLPAFS HGGFSHN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VAP-1. Anti-Human VAP-1-specific antibody was purified by affinity chromatography employing an immobilized Human VAP-1 matrix.
Sandwich ELISA: To detect Human VAP-1 by sandwich ELISA (using 100 µL/well), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Rabbit Anti-Human VAP-1 (500-P326BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VAP-1.
Western Blot: To detect Human VAP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human VAP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been discribed and is located approximately 9-kb downstream.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amine oxidase [copper-containing] 3; Amine oxidase copper-containing 3; amine oxidase, copper containing 3 (vascular adhesion protein 1); Copper amine oxidase; HPAO; placenta copper monamine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; VAP-1; Vascular adhesion protein 1
Gene Aliases: AOC3; HPAO; SSAO; VAP-1; VAP1
UniProt ID: (Human) Q16853
Entrez Gene ID: (Human) 8639
copper ion binding
calcium ion binding
protein binding
primary amine oxidase activity
protein homodimerization activity
protein heterodimerization activity
quinone binding
tryptamine:oxygen oxidoreductase (deaminating) activity
aminoacetone:oxygen oxidoreductase(deaminating) activity
aliphatic-amine oxidase activity
phenethylamine:oxygen oxidoreductase (deaminating) activity
oxidase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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