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Eotaxin Antibody (500-P41BT-1MG) in ELISA
Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Eotaxin Polyclonal Antibody, Biotin (Product # 500-P41BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Eotaxin Polyclonal Antibody (Product # 500-P41-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEotaxin.
Product Details
500-P41BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Eotaxin (CCL11)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930524
Product Specific Information
AA Sequence of recombinant protein: GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Eotaxin (CCL11). Anti-Human Eotaxin (CCL11)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Eotaxin (500-P41) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEotaxin.
Western Blot: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P41BT-1MG will be provided as 2 x 500 µg
Target Information
This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil specific chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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