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Invitrogen
Thrombopoietin Polyclonal Antibody, Biotin, PeproTech®
FIGURE: 1 / 3
Thrombopoietin Antibody (500-P49GBT-1MG) in WB
Western Blot: To detect hTPO by Western Blot analysis Thrombopoietin Polyclonal Antibody, Biotin (Product # 500-P49GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P49GBT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 18.6 kDa Recombinant Human TPO
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930590
Product Specific Information
AA Sequence of recombinant protein: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human TPO. Anti-Human TPO-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TPO (500-P49G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTPO.
Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P49GBT-1MG will be provided as 2 x 500 µg
Target Information
Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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