Invitrogen

CCL3 Polyclonal Antibody, PeproTech®

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View all (37) CCL3 (MIP-1 alpha) antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

500-P77-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
View 4 publications 4 publications

Immunostaining (IS)

-
View 3 publications 3 publications
Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat MIP-1alpha (CCL3)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930756

Product Specific Information

AA Sequence of recombinant protein: APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat MIP-1alpha (CCL3). Anti-Rat MIP-1alpha (CCL3)-specific antibody was purified by affinity chromatography employing an immobilized Rat MIP-1alpha (CCL3) matrix.

Sandwich ELISA: To detect Rat MIP-1alpha (CCL3) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat MIP-1alpha (CCL3) (500-P77Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat MIP-1alpha (CCL3).

Western Blot: To detect Rat MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 3; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; RP23-320E6.7; small inducible cytokine A3; Small-inducible cytokine A3

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Gene Aliases: Ccl3; MIP-1a; Mip1a; Scya3

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UniProt ID: (Rat) P50229

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Entrez Gene ID: (Rat) 25542

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Function(s)
protein kinase activity calcium-dependent protein kinase C activity chemokine activity heparin binding phospholipase activator activity kinase activity CCR1 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity identical protein binding cytokine intercellular signal molecule
Process(es)
MAPK cascade osteoblast differentiation cell activation monocyte chemotaxis protein phosphorylation calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response cytoskeleton organization positive regulation of cytosolic calcium ion concentration cell-cell signaling regulation of cell shape response to toxic substance positive regulation of gene expression negative regulation of gene expression T cell chemotaxis release of sequestered calcium ion into cytosol by sarcoplasmic reticulum calcium-mediated signaling signaling positive regulation of cell migration negative regulation of bone mineralization neutrophil chemotaxis leukocyte chemotaxis lipopolysaccharide-mediated signaling pathway positive regulation of tumor necrosis factor production response to drug positive regulation of catalytic activity eosinophil degranulation protein kinase B signaling positive regulation of neuron apoptotic process astrocyte cell migration negative regulation by host of viral transcription negative regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response positive regulation of calcium-mediated signaling positive chemotaxis positive regulation of protein kinase B signaling positive regulation of calcium ion transport regulation of sensory perception of pain cell chemotaxis positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to organic cyclic compound granulocyte chemotaxis positive regulation of natural killer cell chemotaxis
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