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Invitrogen
CXCL12 beta (SDF-1 beta) Polyclonal Antibody, Biotin, PeproTech®
FIGURE: 1 / 3
CXCL12 beta (SDF-1 beta) Antibody (500-P87BGBT-1MG) in WB
Western Blot: To detect Human SDF-1beta by Western Blot analysis CXCL12 beta (SDF-1 beta) Polyclonal Antibody, Biotin (Product # 500-P87BGBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human SDF-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P87BGBT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 7.9 kDa Recombinant Human SDF-1beta (CXCL12)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930830
Product Specific Information
AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human SDF-1beta (CXCL12). Anti-Human SDF-1beta (CXCL12)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human SDF-1beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SDF-1beta (500-P87BG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human SDF-1beta.
Western Blot: To detect Human SDF-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human SDF-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P87BGBT-1MG will be provided as 2 x 500 µg
Target Information
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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