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Invitrogen

Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor™ 488, eBioscience™

Name: Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor™ 488, eBioscience™
Brand: Invitrogen
SKU: 53-5698-82
Price: 447.0 USD
Availability: InStock

Advanced Verification

74 Published Figures

76 References

View all (225) Ki-67 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

Ki-67 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 83

Ki-67 Antibody (53-5698-82) in ICC/IF

Immunofluorescence analysis of Ki-67 was performed using 70% confluent log phase HeLa cells serum starved (SS) for 36 hours followed by serum release (SR) for 6 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor 488, eBioscience™ (Product # 53-5698-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight. (Panel a: Gr... View More

Ki-67 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ki-67 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Ki-67 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))

Ki-67 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Ki-67 Antibody in Immunohistochemistry (IHC)

Ki-67 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Ki-67 Antibody — Detection of altered target protein expression by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor 488, eBioscience™ (Product # 53-5698-82) shows upregulation of Ki-67 in the nucleus in HeLa cells that were serum starved for 36 hours followed by serum release for 6 hours. The same treatment shows no signal without serum release. {TM}

Product Details

53-5698-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 30 publications 30 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 27 publications 27 publications

Flow Cytometry (Flow)

0.25 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SolA15

Conjugate

Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2802330

Product Specific Information

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).

The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.

Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Refer to BestProtocols® for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Poly-L-ornithine blocks the inhibitory effects of fibronectin on oligodendrocyte differentiation and promotes myelin repair.

Neural regeneration research

Xiong YJ,Soomro SH,Huang ZH,Yu PP,Ping J,Fu H

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Sat Apr 01 00:00:00 UTC 2023

Transformation of primary murine peritoneal mast cells by constitutive KIT activation is accompanied by loss of Cdkn2a/Arf expression.

Frontiers in immunology

Capellmann S,Sonntag R,Schüler H,Meurer SK,Gan L,Kauffmann M,Horn K,Königs-Werner H,Weiskirchen R,Liedtke C,Huber M

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Wed Apr 19 00:00:00 UTC 2023

B-cell antigen receptor expression and phosphatidylinositol 3-kinase signaling regulate genesis and maintenance of mouse chronic lymphocytic leukemia.

Haematologica

Schmid VK,Khadour A,Ahmed N,Brandl C,Nitschke L,Rajewsky K,Jumaa H,Hobeika E

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2022

Inhibition of LncRNA Vof-16 expression promotes nerve regeneration and functional recovery after spinal cord injury.

Neural regeneration research

Zhang XM,Zeng LN,Yang WY,Ding L,Chen KZ,Fu WJ,Zeng SQ,Liang YR,Chen GH,Wu HF

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Sat Jan 01 00:00:00 UTC 2022

Application of an instructive hydrogel accelerates re-epithelialization of xenografted human skin wounds.

Scientific reports

Sparks HD,Mandla S,Vizely K,Rosin N,Radisic M,Biernaskie J

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Sat Aug 20 00:00:00 UTC 2022

Application of an instructive hydrogel accelerates re-epithelialization of xenografted human skin wounds.

Scientific reports

Sparks HD,Mandla S,Vizely K,Rosin N,Radisic M,Biernaskie J

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Sat Aug 20 00:00:00 UTC 2022

Visualization of individual cell division history in complex tissues using iCOUNT.

Cell stem cell

Denoth-Lippuner A,Jaeger BN,Liang T,Royall LN,Chie SE,Buthey K,Machado D,Korobeynyk VI,Kruse M,Munz CM,Gerbaulet A,Simons BD,Jessberger S

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Thu Nov 04 00:00:00 UTC 2021

Epicardial placement of human MSC-loaded fibrin sealant films for heart failure: Preclinical efficacy and mechanistic data.

Molecular therapy : the journal of the American Society of Gene Therapy

Fields L,Ito T,Kobayashi K,Ichihara Y,Podaru MN,Hussain M,Yamashita K,Machado V,Lewis-McDougall F,Suzuki K

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Wed Aug 04 00:00:00 UTC 2021

Environmental oxygen regulates astrocyte proliferation to guide angiogenesis during retinal development.

Development (Cambridge, England)

Perelli RM,O'Sullivan ML,Zarnick S,Kay JN

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Sat May 01 00:00:00 UTC 2021

HDAC1 Regulates Neuronal Differentiation.

Frontiers in molecular neuroscience

Nieto-Estevez V,Changarathil G,Adeyeye AO,Coppin MO,Kassim RS,Zhu J,Hsieh J

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry (PFA fixed)

Tue Feb 01 00:00:00 UTC 2022

Macrophage LAMTOR1 Deficiency Prevents Dietary Obesity and Insulin Resistance Through Inflammation-Induced Energy Expenditure.

Frontiers in cell and developmental biology

Ying L,Zhang M,Ma X,Si Y,Li X,Su J,Yin J,Bao Y

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Tue Jun 08 00:00:00 UTC 2021

Transcription factor old astrocyte specifically induced substance is a novel regulator of kidney fibrosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yamamoto A,Morioki H,Nakae T,Miyake Y,Harada T,Noda S,Mitsuoka S,Matsumoto K,Tomimatsu M,Kanemoto S,Tanaka S,Maeda M,Conway SJ,Imaizumi K,Fujio Y,Obana M

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Mon Feb 01 00:00:00 UTC 2021

HOXA11 plays critical roles in disease progression and response to cytarabine in AML.

Oncology reports

Fu JF,Shih LY,Yen TH

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Thu Jul 01 00:00:00 UTC 2021

Thrombospondin-2 spatiotemporal expression in skeletal fractures.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Zondervan RL,Jenkins DC,Reicha JD,Hankenson KD

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Fri Jan 01 00:00:00 UTC 2021

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.

Cytotechnology

Zeng X,Liao H,Wang F

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Thu Apr 01 00:00:00 UTC 2021

A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice.

Med (New York, N.Y.)

Silva-Cayetano A,Foster WS,Innocentin S,Belij-Rammerstorfer S,Spencer AJ,Burton OT,Fra-Bidó S,Le Lee J,Thakur N,Conceicao C,Wright D,Barrett J,Evans-Bailey N,Noble C,Bailey D,Liston A,Gilbert SC,Lambe T,Linterman MA

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Fri Mar 12 00:00:00 UTC 2021

Adult neural stem cells have latent inflammatory potential that is kept suppressed by Tcf4 to facilitate adult neurogenesis.

Science advances

Shariq M,Sahasrabuddhe V,Krishna S,Radha S,Bellampalli R,Dwivedi A,Cheramangalam R,Reizis B,Hébert J,Ghosh HS

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Sat May 01 00:00:00 UTC 2021

IL-1beta promotes the age-associated decline of beta cell function.

iScience

Böni-Schnetzler M,Méreau H,Rachid L,Wiedemann SJ,Schulze F,Trimigliozzi K,Meier DT,Donath MY

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Fri Nov 19 00:00:00 UTC 2021

Targeting Feedforward Loops Formed by Nuclear Receptor RORγ and Kinase PBK in mCRPC with Hyperactive AR Signaling.

Cancers

Zhang X,Huang Z,Wang J,Ma Z,Yang J,Corey E,Evans CP,Yu AM,Chen HW

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Thu Apr 01 00:00:00 UTC 2021

Requirement of DNMT1 to orchestrate epigenomic reprogramming for NPM-ALK-driven lymphomagenesis.

Life science alliance

Redl E,Sheibani-Tezerji R,Cardona CJ,Hamminger P,Timelthaler G,Hassler MR,Zrimšek M,Lagger S,Dillinger T,Hofbauer L,Draganić K,Tiefenbacher A,Kothmayer M,Dietz CH,Ramsahoye BH,Kenner L,Bock C,Seiser C,Ellmeier W,Schweikert G,Egger G

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Mon Feb 01 00:00:00 UTC 2021

Notch Signaling between Cerebellar Granule Cell Progenitors.

eNeuro

Adachi T,Miyashita S,Yamashita M,Shimoda M,Okonechnikov K,Chavez L,Kool M,Pfister SM,Inoue T,Kawauchi D,Hoshino M

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Fri Jul 02 00:00:00 UTC 2021

Delta-like 4 is required for pulmonary vascular arborization and alveolarization in the developing lung.

JCI insight

Xia S,Menden HL,Townley N,Mabry SM,Johnston J,Nyp MF,Heruth DP,Korfhagen T,Sampath V

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Thu Apr 08 00:00:00 UTC 2021

Dissecting phenotypic transitions in metastatic disease via photoconversion-based isolation.

eLife

Sela Y,Li J,Kuri P,Merrell AJ,Li N,Lengner C,Rompolas P,Stanger BZ

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Tue Feb 23 00:00:00 UTC 2021

Cell death & disease

Catlin JP,Marziali LN,Rein B,Yan Z,Feltri ML,Schaner Tooley CE

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Thu Oct 28 00:00:00 UTC 2021

CDCP1 promotes compensatory renal growth by integrating Src and Met signaling.

Life science alliance

Kajiwara K,Yamano S,Aoki K,Okuzaki D,Matsumoto K,Okada M

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry (PFA fixed)

Thu Apr 01 00:00:00 UTC 2021

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

Shikonin Derivatives from Onsoma visianii Decrease Expression of Phosphorylated STAT3 in Leukemia Cells and Exert Antitumor Activity.

Nutrients

Todorovic Z,Milovanovic J,Arsenijevic D,Vukovic N,Vukic M,Arsenijevic A,Djurdjevic P,Milovanovic M,Arsenijevic N

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Wed Mar 31 00:00:00 UTC 2021

An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia.

Cell reports

Suzuki S,McCarrey JR,Hermann BP

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Tue Feb 16 00:00:00 UTC 2021

An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia.

Cell reports

Suzuki S,McCarrey JR,Hermann BP

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Tue Feb 16 00:00:00 UTC 2021

Ten-eleven translocation protein 1 modulates medulloblastoma progression.

Genome biology

Kim H,Kang Y,Li Y,Chen L,Lin L,Johnson ND,Zhu D,Robinson MH,McSwain L,Barwick BG,Yuan X,Liao X,Zhao J,Zhang Z,Shu Q,Chen J,Allen EG,Kenney AM,Castellino RC,Van Meir EG,Conneely KN,Vertino PM,Jin P,Li J

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Thu Apr 29 00:00:00 UTC 2021

TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression.

Cells

Kwon O,Yang H,Kim SC,Kim J,Sim J,Lee J,Hwang EM,Shim S,Park JY

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Thu Oct 14 00:00:00 UTC 2021

GTSE1 is possibly involved in the DNA damage repair and cisplatin resistance in osteosarcoma.

Journal of orthopaedic surgery and research

Xie C,Xiang W,Shen H,Shen J

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Tue Dec 07 00:00:00 UTC 2021

CXCR4 Regulates Temporal Differentiation via PRC1 Complex in Organogenesis of Epithelial Glands.

International journal of molecular sciences

Kim J,Lee SW,Park K

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Sun Jan 10 00:00:00 UTC 2021

Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams.

Scientific reports

Ojima M,Ito A,Usami N,Ohara M,Suzuki K,Kai M

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Fri Mar 26 00:00:00 UTC 2021

Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway.

Nature communications

Fujino T,Goyama S,Sugiura Y,Inoue D,Asada S,Yamasaki S,Matsumoto A,Yamaguchi K,Isobe Y,Tsuchiya A,Shikata S,Sato N,Morinaga H,Fukuyama T,Tanaka Y,Fukushima T,Takeda R,Yamamoto K,Honda H,Nishimura EK,Furukawa Y,Shibata T,Abdel-Wahab O,Suematsu M,Kitamura T

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Tue Mar 23 00:00:00 UTC 2021

NEIL3-deficiency increases gut permeability and contributes to a pro-atherogenic metabolic phenotype.

Scientific reports

Karlsen TR,Kong XY,Holm S,Quiles-Jiménez A,Dahl TB,Yang K,Sagen EL,Skarpengland T,S Øgaard JD,Holm K,Vestad B,Olsen MB,Aukrust P,Bjørås M,Hov JR,Halvorsen B,Gregersen I

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Tue Oct 05 00:00:00 UTC 2021

Gene Signatures Detect Damaged Liver Sinusoidal Endothelial Cells in Chronic Liver Diseases.

Frontiers in medicine

Verhulst S,van Os EA,De Smet V,Eysackers N,Mannaerts I,van Grunsven LA

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Wed Nov 10 00:00:00 UTC 2021

Developmental Role of Adenosine Kinase in the Cerebellum.

eNeuro

Gebril H,Wahba A,Zhou X,Lai T,Alharfoush E,DiCicco-Bloom E,Boison D

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Fri Jul 02 00:00:00 UTC 2021

Imbalanced Activation of Wnt-/β-Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation.

Frontiers in physiology

Koch PS,Sandorski K,Heil J,Schmid CD,Kürschner SW,Hoffmann J,Winkler M,Staniczek T,de la Torre C,Sticht C,Schledzewski K,Taketo MM,Trogisch FA,Heineke J,Géraud C,Goerdt S,Olsavszky V

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Fri Oct 22 00:00:00 UTC 2021

An in vivo model allowing continuous observation of human vascular formation in the same animal over time.

Scientific reports

Tsukada Y,Muramatsu F,Hayashi Y,Inagaki C,Su H,Iba T,Kidoya H,Takakura N

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Tue Jan 12 00:00:00 UTC 2021

Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.

Cell stem cell

Katsura H,Sontake V,Tata A,Kobayashi Y,Edwards CE,Heaton BE,Konkimalla A,Asakura T,Mikami Y,Fritch EJ,Lee PJ,Heaton NS,Boucher RC,Randell SH,Baric RS,Tata PR

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Thu Dec 03 00:00:00 UTC 2020

Egr2-guided histone H2B monoubiquitination is required for peripheral nervous system myelination.

Nucleic acids research

Wüst HM,Wegener A,Fröb F,Hartwig AC,Wegwitz F,Kari V,Schimmel M,Tamm ER,Johnsen SA,Wegner M,Sock E

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Fri Sep 18 00:00:00 UTC 2020

Genetic manipulation of LKB1 elicits lethal metastatic prostate cancer.

The Journal of experimental medicine

Hermanova I,Zúñiga-García P,Caro-Maldonado A,Fernandez-Ruiz S,Salvador F,Martín-Martín N,Zabala-Letona A,Nuñez-Olle M,Torrano V,Camacho L,Lizcano JM,Talamillo A,Carreira S,Gurel B,Cortazar AR,Guiu M,López JI,Martinez-Romero A,Astobiza I,Valcarcel-Jimenez L,Lorente M,Arruabarrena-Aristorena A,Velasco G,Gomez-Muñoz A,Suárez-Cabrera C,Lodewijk I,Flores JM,Sutherland JD,Barrio R,de Bono JS,Paramio JM,Trka J,Graupera M,Gomis RR,Carracedo A

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Mon Jun 01 00:00:00 UTC 2020

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Developmental cell

Vila Ellis L,Cain MP,Hutchison V,Flodby P,Crandall ED,Borok Z,Zhou B,Ostrin EJ,Wythe JD,Chen J

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Mon Mar 09 00:00:00 UTC 2020

Multimodal single-cell analysis reveals distinct radioresistant stem-like and progenitor cell populations in murine glioma.

Glia

Alexander J,LaPlant QC,Pattwell SS,Szulzewsky F,Cimino PJ,Caruso FP,Pugliese P,Chen Z,Chardon F,Hill AJ,Spurrell C,Ahrendsen D,Pietras A,Starita LM,Hambardzumyan D,Iavarone A,Shendure J,Holland EC

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Tue Dec 01 00:00:00 UTC 2020

FGF2 modulates simultaneously the mode, the rate of division and the growth fraction in cultures of radial glia.

Development (Cambridge, England)

Ledesma-Terrón M,Peralta-Cañadas N,Míguez DG

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Fri Jul 24 00:00:00 UTC 2020

NPC1 deficiency impairs cerebellar postnatal development of microglia and climbing fiber refinement in a mouse model of Niemann-Pick disease type C.

Development (Cambridge, England)

Boyle BR,Melli SE,Altreche RS,Padron ZM,Yousufzai FAK,Kim S,Vasquez MD,Carone DM,Carone BR,Soto I

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry (Frozen)

Mon Aug 03 00:00:00 UTC 2020

Novel enhanced GFP-positive congenic inbred strain establishment and application of tumor-bearing nude mouse model.

Cancer science

Lan Q,Chen Y,Dai C,Li S,Fei X,Dong J,Shen Y,Dai X,Lu Z,Liu B,Wang Q,Wang H,Zhou Z,Ji X,Wang Z,Huang Q

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Thu Oct 01 00:00:00 UTC 2020

Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis.

Journal of leukocyte biology

Rodrigues TS,Alvarez ARP,Gembre AF,Forni MFPAD,de Melo BMS,Alves Filho JCF,Câmara NOS,Bonato VLD

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2020

Radiation Induced Metabolic Alterations Associate With Tumor Aggressiveness and Poor Outcome in Glioblastoma.

Frontiers in oncology

Gupta K,Vuckovic I,Zhang S,Xiong Y,Carlson BL,Jacobs J,Olson I,Petterson XM,Macura SI,Sarkaria J,Burns TC

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Mon Sep 28 00:00:00 UTC 2020

Radiation Induced Metabolic Alterations Associate With Tumor Aggressiveness and Poor Outcome in Glioblastoma.

Frontiers in oncology

Gupta K,Vuckovic I,Zhang S,Xiong Y,Carlson BL,Jacobs J,Olson I,Petterson XM,Macura SI,Sarkaria J,Burns TC

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Mon Sep 28 00:00:00 UTC 2020

Agrin Promotes Limbal Stem Cell Proliferation and Corneal Wound Healing Through Hippo-Yap Signaling Pathway.

Investigative ophthalmology & visual science

Hou L,Fu W,Liu Y,Wang Q,Wang L,Huang Y

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Mon May 11 00:00:00 UTC 2020

New Insights into the Pathogenesis of Diabetic Nephropathy: Proximal Renal Tubules Are Primary Target of Oxidative Stress in Diabetic Kidney.

Acta histochemica et cytochemica

Haraguchi R,Kohara Y,Matsubayashi K,Kitazawa R,Kitazawa S

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Tue Apr 28 00:00:00 UTC 2020

A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain.

eLife

Matsumoto N,Tanaka S,Horiike T,Shinmyo Y,Kawasaki H

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Tue Apr 21 00:00:00 UTC 2020

A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain.

eLife

Matsumoto N,Tanaka S,Horiike T,Shinmyo Y,Kawasaki H

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Tue Apr 21 00:00:00 UTC 2020

Treatment of mice with a ligand binding blocking anti-CD28 monoclonal antibody improves healing after myocardial infarction.

PloS one

Gladow N,Hollmann C,Ramos G,Frantz S,Kerkau T,Beyersdorf N,Hofmann U

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Mon Jul 06 00:00:00 UTC 2020

Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.

Nature communications

Riedel R,Addo R,Ferreira-Gomes M,Heinz GA,Heinrich F,Kummer J,Greiff V,Schulz D,Klaeden C,Cornelis R,Menzel U,Kröger S,Stervbo U,Köhler R,Haftmann C,Kühnel S,Lehmann K,Maschmeyer P,McGrath M,Naundorf S,Hahne S,Sercan-Alp Ö,Siracusa F,Stefanowski J,Weber M,Westendorf K,Zimmermann J,Hauser AE,Reddy ST,Durek P,Chang HD,Mashreghi MF,Radbruch A

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Fri May 22 00:00:00 UTC 2020

A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons.

eLife

Okaty BW,Sturrock N,Escobedo Lozoya Y,Chang Y,Senft RA,Lyon KA,Alekseyenko OV,Dymecki SM

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Mon Jun 22 00:00:00 UTC 2020

Interleukin-13 disrupts type 2 pneumocyte stem cell activity.

JCI insight

Glisinski KM,Schlobohm AJ,Paramore SV,Birukova A,Moseley MA,Foster MW,Barkauskas CE

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Thu Jan 16 00:00:00 UTC 2020

Interleukin-13 disrupts type 2 pneumocyte stem cell activity.

JCI insight

Glisinski KM,Schlobohm AJ,Paramore SV,Birukova A,Moseley MA,Foster MW,Barkauskas CE

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Thu Jan 16 00:00:00 UTC 2020

Elevated Serum Amino Acids Induce a Subpopulation of Alpha Cells to Initiate Pancreatic Neuroendocrine Tumor Formation.

Cell reports. Medicine

Smith DK,Kates L,Durinck S,Patel N,Stawiski EW,Kljavin N,Foreman O,Sipos B,Solloway MJ,Allan BB,Peterson AS

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Tue Aug 25 00:00:00 UTC 2020

Changes in Liver Mechanical Properties and Water Diffusivity During Normal Pregnancy Are Driven by Cellular Hypertrophy.

Frontiers in physiology

Garczyńska K,Tzschätzsch H,Kühl AA,Morr AS,Lilaj L,Häckel A,Schellenberger E,Berndt N,Holzhütter HG,Braun J,Sack I,Guo J

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Fri Dec 18 00:00:00 UTC 2020

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Wed Dec 02 00:00:00 UTC 2020

Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2.

Science advances

Zhao G,Weiner AI,Neupauer KM,de Mello Costa MF,Palashikar G,Adams-Tzivelekidis S,Mangalmurti NS,Vaughan AE

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Sun Nov 01 00:00:00 UTC 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Thu Dec 17 00:00:00 UTC 2020

Aripiprazole reduces liver cell division.

PloS one

Pirc Marolt T,Kramar B,Bulc Rozman K,Šuput D,Milisav I

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Tue Dec 08 00:00:00 UTC 2020

Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model.

International journal of molecular sciences

Yoon CH,Ryu JS,Hwang HS,Kim MK

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Thu Jun 11 00:00:00 UTC 2020

Transcriptome analysis of basic fibroblast growth factor treated stem cells isolated from human exfoliated deciduous teeth.

Heliyon

Nowwarote N,Manokawinchoke J,Kanjana K,Fournier BPJ,Sukarawan W,Osathanon T

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Mon Jun 01 00:00:00 UTC 2020

The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis.

BMC research notes

Cousins FL,Farley JK,Kerrigan R,Mukherjee S,Darzi S,Gargett CE,Deane JA

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Fri Sep 25 00:00:00 UTC 2020

Metastasis-initiating cells induce and exploit a fibroblast niche to fuel malignant colonization of the lungs.

Nature communications

Pein M,Insua-Rodríguez J,Hongu T,Riedel A,Meier J,Wiedmann L,Decker K,Essers MAG,Sinn HP,Spaich S,Sütterlin M,Schneeweiss A,Trumpp A,Oskarsson T

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Fri Mar 20 00:00:00 UTC 2020

B Cell Activating Factor (BAFF) Is Required for the Development of Intra-Renal Tertiary Lymphoid Organs in Experimental Kidney Transplantation in Rats.

International journal of molecular sciences

Steines L,Poth H,Herrmann M,Schuster A,Banas B,Bergler T

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Wed Oct 28 00:00:00 UTC 2020

Microbiota-Sourced Purines Support Wound Healing and Mucous Barrier Function.

iScience

Lee JS,Wang RX,Goldberg MS,Clifford GP,Kao DJ,Colgan SP

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry (Paraffin)

Fri Jun 26 00:00:00 UTC 2020

Eradication of tumor growth by delivering novel photothermal selenium-coated tellurium nanoheterojunctions.

Science advances

Chen S,Xing C,Huang D,Zhou C,Ding B,Guo Z,Peng Z,Wang D,Zhu X,Liu S,Cai Z,Wu J,Zhao J,Wu Z,Zhang Y,Wei C,Yan Q,Wang H,Fan D,Liu L,Zhang H,Cao Y

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Wed Apr 01 00:00:00 UTC 2020

Deficiency in the secreted protein Semaphorin3d causes abnormal parathyroid development in mice.

The Journal of biological chemistry

Singh A,Mia MM,Cibi DM,Arya AK,Bhadada SK,Singh MK

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunocytochemistry

Fri May 24 00:00:00 UTC 2019

Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.

Cell stem cell

Tata A,Kobayashi Y,Chow RD,Tran J,Desai A,Massri AJ,McCord TJ,Gunn MD,Tata PR

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Immunohistochemistry

Thu May 03 00:00:00 UTC 2018

De Novo Epigenetic Programs Inhibit PD-1 Blockade-Mediated T Cell Rejuvenation.

Cell

Ghoneim HE,Fan Y,Moustaki A,Abdelsamed HA,Dash P,Dogra P,Carter R,Awad W,Neale G,Thomas PG,Youngblood B

Published figure using Ki-67 monoclonal antibody (Product # 53-5698-82) in Flow Cytometry

Thu Jun 29 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; unnamed protein product

Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

UniProt ID: (Human) P46013, (Mouse) E9PVX6

Entrez Gene ID: (Dog) 100686578, (Human) 4288, (Cynomolgus monkey) 102135895, (Rat) 291234, (Mouse) 17345

Function(s)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function

Process(es)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor™ 488, eBioscience™

Ki-67 Antibody (53-5698-82) in ICC/IF

Product Details

53-5698-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Recommended Isotype Control

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Type

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Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

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Bioinformatics

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Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor™ 488, eBioscience™

Ki-67 Antibody (53-5698-82) in ICC/IF

Product Details

53-5698-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

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Bioinformatics

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We're here to help

Your items have has been added!