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Invitrogen
6x-His Tag Monoclonal Antibody (HIS.H8), DyLight™ 680
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Application
FIGURE: 1 / 9
6x-His Tag Antibody (MA1-21315-D680) in ICC/IF
Immunofluorescent analysis of 6x-His Tag was performed using 70% confluent HEK-293 cells transfected with V5-H3-His construct. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 6x-His Tag Monoclonal Antibody (HIS.H8), DyLight 680 (Product # MA1-21315-D680) at ... View More
Product Details
MA1-21315-D680
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
HIS.H8
Immunogen
6x His synthetic peptide.
Conjugate
DyLight™ 680
Excitation/Emission Max
676/705 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536987
Product Specific Information
MA1-21315-D680 has been successfully used in immunofluorescence and Western blot applications. DyLight 680 has an excitation/emission of 692/712 nm.
Target Information
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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