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            • Primary Antibodies ›
            • 6x His Tag Antibodies

            Invitrogen

            6x-His Tag (C-term) Monoclonal Antibody (3D5), HRP

            11 Published Figures
            38 References
            View all (135) 6x His Tag antibodies
            Datasheet
            Protocols
            Questions & Answers
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            Catalog # R931-25
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            Price (USD)
            424.00
            50 µL
             
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            Datasheet
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            Cite 6x-His Tag (C-term) Monoclonal Antibody (3D5), HRP

            • Antibody Testing Data (1)
            • Published Figures (11)
            Application
            6x-His Tag (C-term) Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 12

            6x-His Tag (C-term) Antibody (R931-25) in WB

            Western blot analysis of Histidine tag (6X His) was performed by loading 20 µg of whole cell extracts of untransfected HEK-293 and HEK-293 transiently overexpressing His-IGFBP6 using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), Xcell SureLock Electrophoresis system (Product # EI0002), Novex Sharp Pre-stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. His-IGFBP6 was detected at ~31 kDa us... View More
            View Product

            6x-His Tag (C-term) Antibody in Western Blot (WB)
            6x-His Tag (C-term) Antibody in Western Blot (WB)
            6x-His Tag (C-term) Antibody in Western Blot (WB)
            6x-His Tag (C-term) Antibody in Western Blot (WB)
            6x-His Tag (C-term) Antibody in Western Blot (WB)
            6x-His Tag (C-term) Antibody in Western Blot (WB)
            6x-His Tag (C-term) Antibody in Western Blot (WB)
            6x-His Tag (C-term) Antibody in Western Blot (WB)
            6x-His Tag (C-term) Antibody in Immunocytochemistry (ICC/IF)
            6x-His Tag (C-term) Antibody in Immunoprecipitation (IP)
            6x-His Tag (C-term) Antibody in ELISA (ELISA)
            6x-His Tag (C-term) Antibody in Peptide array (ARRAY)

            Product Details

            R931-25

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000-1:5,000
            View 23 publications 23 publications

            Immunocytochemistry (ICC/IF)

            -
            View 1 publication 1 publication

            ELISA (ELISA)

            -
            View 8 publications 8 publications

            Immunoprecipitation (IP)

            -
            View 2 publications 2 publications

            Functional Assay (Functional)

            -
            View 1 publication 1 publication

            Peptide Array (Array)

            -
            View 1 publication 1 publication

            Immunoelectrophoresis (IE)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Tag

            Published species

            Tag

            Host/Isotype

            Mouse / IgG2b

            Class

            Monoclonal

            Type

            Antibody

            Clone

            3D5

            Immunogen

            C-term 6x His synthetic peptide: -His-His-His-His-His-His-COOH

            Conjugate

            HRP
            • Unconjugated
            • AP
            • FITC
            • Request custom conjugation

            Form

            Liquid

            Purification

            purified

            Storage buffer

            PBS

            Contains

            no preservative

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            RRID

            AB_2556554

            Product Specific Information

            NOTE: R931-25 was previously sold under product #46-0707. When re-ordering, please use #R931-25.

            This antibody can be used to detect expression of C-terminal His fusion proteins from bacterial, insect, and mammalian cells.

            This product contains enough material for 25 Western blots. It is functionally tested using 100 ng of an E. coli expressed fusion protein containing a C-terminal polyhistidine (6xHis) epitope. Chemiluminescent detection should be performed using a 1 minute exposure. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM).

            Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.

            Target Information

            The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            It has to be done as per old AB suggested Products section.
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