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Invitrogen
A33 Recombinant Rabbit Monoclonal Antibody (JE49-54)
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Application
FIGURE: 1 / 5
A33 Antibody (MA5-34867) in IHC (P)
Immunohistochemistry analysis of GPA33 in paraffin-embedded human small intestine tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with GPA33 monoclonal antibody (Product # MA5-34867) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with... View More
Product Details
MA5-34867
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE49-54
Immunogen
Recombinant protein within Human GPA33 aa 203-319
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848775
Product Specific Information
Positive Control: Human small intestine tissue, human colon tissue, human colon cancer tissue, human appendix tissue.
Target Information
The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell surface A33 antigen; Glycoprotein A33; glycoprotein A33 (transmembrane); MGC129986; MGC129987; transmembrane glycoprotein A33
Gene Aliases: A33; GPA33
UniProt ID: (Human) Q99795
Entrez Gene ID: (Human) 10223
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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