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Invitrogen

ABCA1 Monoclonal Antibody (3A1.891.3)

Name: ABCA1 Monoclonal Antibody (3A1.891.3)
Brand: Invitrogen
SKU: MA116936
Price: 550.0 USD

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1 Reference

View all (19) ABCA1 antibodies

Datasheet

Protocols

Questions & Answers

Catalog # MA1-16936

Price (USD)

550.00

100 µL

Estimated availability date: 16-Jun-2025

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (1)

Application

ABCA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

ABCA1 Antibody (MA1-16936) in ICC/IF

Immunofluorescence analysis of ABCA1 was performed using log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ABCA1 Monoclonal Antibody (3A1.891.3) (Product # MA1-16936) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006) at a dilution of 1:2000 f... View More

ABCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

ABCA1 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using ABCA1 Monoclonal Antibody (3A1.891.3) (Product # MA1-16936) shows an increase in expression of ABCA1 in THP-1 upon LPS treatment. {TM}

Product Details

MA1-16936

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3A1.891.3

Immunogen

Synthetic peptide corrseponding to the N-terminal loop residues 200-250 of mouse ABCA1.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_779127

Product Specific Information

This antibody does not react with human ABCA1.

Target Information

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enhanced liver X receptor signalling reduces brain injury and promotes tissue regeneration following experimental intracerebral haemorrhage: roles of microglia/macrophages.

Stroke and vascular neurology

Zhang R,Dong Y,Liu Y,Moezzi D,Ghorbani S,Mirzaei R,Lozinski BM,Dunn JF,Yong VW,Xue M

MA1-16936 was used in Immunohistochemistry-immunofluorescence to examine the benefits of enhanced Liver X receptor signalling in experimental intracerebral haemorrhage.

Fri Dec 29 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: ABC 1; ABC 1 antibody; ABC Transporter 1; ABC-1; ABC1 antibody; ATP-binding cassette 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; CERP antibody; Phospholipid-transporting ATPase ABCA1

Gene Aliases: ABC-1; Abc1; Abca1

UniProt ID: (Mouse) P41233

Entrez Gene ID: (Mouse) 11303

Function(s)

nucleotide binding receptor binding transporter activity protein binding ATP binding phospholipid transporter activity anion transmembrane transporter activity ATPase activity cholesterol transporter activity syntaxin binding small GTPase binding apolipoprotein binding apolipoprotein A-I binding apolipoprotein A-I receptor activity ATPase activity, coupled to transmembrane movement of substances ATPase binding ATP-binding cassette (ABC) transporter primary active transporter transporter

Process(es)

peptide secretion protein lipidation transport phagocytosis, engulfment lysosome organization G-protein coupled receptor signaling pathway cholesterol metabolic process positive regulation of cholesterol efflux phospholipid transport endosomal transport cholesterol transport positive regulation of cAMP biosynthetic process intracellular cholesterol transport regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux high-density lipoprotein particle assembly apolipoprotein A-I-mediated signaling pathway lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis reverse cholesterol transport phospholipid translocation interleukin-1 beta secretion transmembrane transport phospholipid homeostasis response to high density lipoprotein particle platelet dense granule organization cellular response to lipopolysaccharide cellular response to retinoic acid anion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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