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Invitrogen

ACE2 Monoclonal Antibody (CL4035)

Advanced Verification

6 Published Figures

10 References

View all (72) ACE2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

ACE2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 15

ACE2 Antibody (MA5-31395) in IHC (P)

Immunohistochemical analysis of ACE2 in human kidney using ACE2 Monoclonal Antibody (CL4035) (Product # MA5-31395) shows strong positivity in apical membrane in cells in proximal tubules.

ACE2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ACE2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Angiotensin-converting enzyme 2 was observed in HaCaT, Hep G2 and RT4 but not in BJ and U-2 OS (DOI: 10.3892/mmr.2014.2266) using Anti-ACE2 Monoclonal Antibody (CL4035) (Product # MA5-31395) in Western Blot. {RE}

Product Details

MA5-31395

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20,000-1:50,000

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CL4035

Immunogen

Recombinant protein corresponding to Human ACE2

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2787031

Product Specific Information

Immunogen sequence: MSSSSWLLLS LVAVTAAQST IEEQAKTFLD KFNHEAEDLF YQSSLASWNY NTNITEENVQ NMNNAGDKWS AFLKEQSTLA QMYPLQEIQN LTVKLQLQAL QQNGSSVLSE D

Target Information

Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Significance of Furin Expression in Thyroid Neoplastic Transformation.

Cancers

Azevedo MT,Macedo S,Canberk S,Cardoso L,Gaspar TB,Pestana A,Batista R,Sobrinho-Simões M,Soares P

Published figure using ACE2 monoclonal antibody (Product # MA5-31395) in Immunohistochemistry

Tue Aug 01 00:00:00 UTC 2023

Significance of Furin Expression in Thyroid Neoplastic Transformation.

Cancers

Azevedo MT,Macedo S,Canberk S,Cardoso L,Gaspar TB,Pestana A,Batista R,Sobrinho-Simões M,Soares P

Published figure using ACE2 monoclonal antibody (Product # MA5-31395) in Immunohistochemistry

Tue Aug 01 00:00:00 UTC 2023

IL-9 aggravates SARS-CoV-2 infection and exacerbates associated airway inflammation.

Nature communications

Sadhu S,Dalal R,Dandotiya J,Binayke A,Singh V,Tripathy MR,Das V,Goswami S,Kumar S,Rizvi ZA,Awasthi A

MA5-31395 was used in Western Blot to provide mechanistic insight into an important inflammatory pathway in SARS-CoV-2 infection, and thus represents proof of principle for the development of host-directed therapeutics to mitigate disease severity.

Mon Jul 10 00:00:00 UTC 2023

Cutaneous and systemic hyperinflammation drives maculopapular drug exanthema in severely ill COVID-19 patients.

Allergy

Mitamura Y,Schulz D,Oro S,Li N,Kolm I,Lang C,Ziadlou R,Tan G,Bodenmiller B,Steiger P,Marzano A,de Prost N,Caudin O,Levesque M,Stoffel C,Schmid-Grendelmeier P,Maverakis E,Akdis CA,Brüggen MC

Published figure using ACE2 monoclonal antibody (Product # MA5-31395) in Immunohistochemistry

Tue Feb 01 00:00:00 UTC 2022

Cutaneous and systemic hyperinflammation drives maculopapular drug exanthema in severely ill COVID-19 patients.

Allergy

Mitamura Y,Schulz D,Oro S,Li N,Kolm I,Lang C,Ziadlou R,Tan G,Bodenmiller B,Steiger P,Marzano A,de Prost N,Caudin O,Levesque M,Stoffel C,Schmid-Grendelmeier P,Maverakis E,Akdis CA,Brüggen MC

Published figure using ACE2 monoclonal antibody (Product # MA5-31395) in Immunohistochemistry

Tue Feb 01 00:00:00 UTC 2022

Detection of SARS-CoV-2 infection in thyroid follicular cells from a COVID-19 autopsy series.

European thyroid journal

Macedo S,Pestana A,Santos L,Neves C,Guimarães S,Duarte-Neto A,Dolhnikoff M,Saldiva P,Alves G,Oliveira R,Cabanes D,Carneiro F,Sobrinho-Simões M,Soares P

Published figure using ACE2 monoclonal antibody (Product # MA5-31395) in Immunohistochemistry

Mon Aug 01 00:00:00 UTC 2022

Detection of SARS-CoV-2 infection in thyroid follicular cells from a COVID-19 autopsy series.

European thyroid journal

Macedo S,Pestana A,Santos L,Neves C,Guimarães S,Duarte-Neto A,Dolhnikoff M,Saldiva P,Alves G,Oliveira R,Cabanes D,Carneiro F,Sobrinho-Simões M,Soares P

Published figure using ACE2 monoclonal antibody (Product # MA5-31395) in Immunohistochemistry

Mon Aug 01 00:00:00 UTC 2022

GLP-1 Mediates Regulation of Colonic ACE2 Expression by the Bile Acid Receptor GPBAR1 in Inflammation.

Cells

Biagioli M,Marchianò S,Roselli R,Di Giorgio C,Bellini R,Bordoni M,Distrutti E,Catalanotti B,Zampella A,Graziosi L,Donini A,Fiorucci S

Published figure using ACE2 monoclonal antibody (Product # MA5-31395) in Western Blot

Fri Apr 01 00:00:00 UTC 2022

Hydroxypropyl-beta-cyclodextrin (HP-BCD) inhibits SARS-CoV-2 replication and virus-induced inflammatory cytokines.

Antiviral research

Bezerra BB,Silva GPDD,Coelho SVA,Correa IA,Souza MRM,Macedo KVG,Matos BM,Tanuri A,Matassoli FL,Costa LJD,Hildreth JEK,Arruda LB

MA5-31395 was used in Flow Cytometry to indicate that the use hydroxypropyl-beta-cyclodextrin (HP-BCD), which inhibits both SARS-CoV2 replication and production of proinflammatory cytokines, as a potential COVID-19 therapeutic warrants further investigation.

Thu Sep 01 00:00:00 UTC 2022

InfectionCMA: A Cell MicroArray Approach for Efficient Biomarker Screening in In Vitro Infection Assays.

Pathogens (Basel, Switzerland)

Magalhães AC,Ricardo S,Moreira AC,Nunes M,Tavares M,Pinto RJ,Gomes MS,Pereira L

MA5-31395 was used in Immunocytochemistry to demonstrate that the chip-like organization of the InfectionCMA has several advantages, allowing side-by-side comparisons between diverse cell lines, infection time points, and biomarker expression and cytolocalization evaluation in the same slide.

Thu Mar 03 00:00:00 UTC 2022

Dodging COVID-19 infection: low expression and localization of ACE2 and TMPRSS2 in multiple donor-derived lines of human umbilical cord-derived mesenchymal stem cells.

Journal of translational medicine

Hernandez JJ,Beaty DE,Fruhwirth LL,Lopes Chaves AP,Riordan NH

MA5-31395 was used in Immunocytochemistry to quantify the expression of ACE2 and TMPRSS2 in hUC-MSCs lots derived from multiple donors using molecular-based techniques in order to demonstrate their inability to be a host to SARS-CoV-2.

Wed Apr 14 00:00:00 UTC 2021

Hydrogen Sulfide Inhibits TMPRSS2 in Human Airway Epithelial Cells: Implications for SARS-CoV-2 Infection.

Biomedicines

Pozzi G,Masselli E,Gobbi G,Mirandola P,Taborda-Barata L,Ampollini L,Carbognani P,Micheloni C,Corazza F,Galli D,Carubbi C,Vitale M

MA5-31395 was used in Western Blotting to suggest that inhalational exposure of respiratory epithelial cells to natural H2S sources may hinder SARS-CoV-2 entry into airway epithelial cells and, consequently, potentially prevent the virus from spreading into the lower respiratory tract and the lung.

Mon Sep 20 00:00:00 UTC 2021

Subchronic treatment with fragments of beta-endorphin prevents electroencephalographic seizures and behavioral alterations induced by centrally administered beta-endorphin in the rabbit.

The Journal of pharmacology and experimental therapeutics

Di Giannuario A,Pieretti S,Luzi M,Loizzo A

Published figure using ACE2 monoclonal antibody (Product # MA5-31395) in Immunohistochemistry

Tue Feb 01 00:00:00 UTC 1994

Bioinformatics

Protein Aliases: ACE 2; ACE-related carboxypeptidase; ACEH; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; Angiotensin-converting enzyme-related carboxypeptidase; Metalloprotease MPROT15; peptidyl-dipeptidase A

Gene Aliases: ACE2; ACEH; UNQ868/PRO1885

UniProt ID: (Human) Q9BYF1

Entrez Gene ID: (Human) 59272

Function(s)

virus receptor activity glycoprotein binding endopeptidase activity carboxypeptidase activity metallocarboxypeptidase activity protein binding metallopeptidase activity peptidyl-dipeptidase activity zinc ion binding metalloprotease

Process(es)

regulation of cytokine production angiotensin maturation angiotensin catabolic process in blood angiotensin-mediated drinking behavior regulation of systemic arterial blood pressure by renin-angiotensin tryptophan transport regulation of vasoconstriction receptor biosynthetic process regulation of cell proliferation regulation of vasodilation viral entry into host cell receptor-mediated virion attachment to host cell regulation of inflammatory response positive regulation of cardiac muscle contraction positive regulation of gap junction assembly regulation of cardiac conduction positive regulation of reactive oxygen species metabolic process

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ACE2 Monoclonal Antibody (CL4035)

ACE2 Antibody (MA5-31395) in IHC (P)

Product Details

MA5-31395

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions