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Invitrogen
ADRA2B Polyclonal Antibody
Application
FIGURE: 1 / 1
ADRA2B Antibody (PA5-109364) in WB
Western blot analysis of ADRA2B in extracts of various cell lines. Samples were incubated with ADRA2B Polyclonal antibody (Product # PA5-109364) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-109364
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ADRA2B (NP_000673.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.08 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854775
Product Specific Information
Immunogen sequence: MDHQDPYSVQ ATAAIAAAIT FLILFTIFGN ALVILAVLTS RSLRAPQNLF LVSLAAADIL VATLIIPFSL ANELLGYWYF RRTWCEVYLA LDVLFCTSSI
Target Information
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADA2B; ADRA2B adrenergic, alpha-2B-, receptor; Adrenergic alpha2B- receptor class III; adrenergic receptor, alpha 2b; adrenergic, alpha-2B-, receptor; Adrenergic, alpha2B-, receptor class III; Alpha-2 adrenergic receptor subtype C2; alpha-2-adrenergic receptor-like 1; Alpha-2B adrenergic receptor; Alpha-2B adrenoceptor; Alpha-2B adrenoreceptor; alpha-2BAR; G-protein coupled receptor; subtype alpha2-C2
Gene Aliases: [a]2B; Adra-2b; ADRA2B; ADRA2L1; ADRA2RL1; ADRARL1; alpha-2BAR; alpha2-C2; alpha2B; ALPHA2BAR; FAME2
UniProt ID: (Human) P18089, (Mouse) F8VQ23, (Rat) P19328
Entrez Gene ID: (Human) 151, (Mouse) 11552, (Rat) 24174
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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