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Bethyl Laboratories
AF4 Polyclonal Antibody
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Application
FIGURE: 1 / 1
AF4 Antibody (A302-345A) in IP
Detection of human AF4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF4 antibody (Product # A302-345A) used for IP at 3 µg/mg lysate. AF4 was also immunoprecipitated by rabbit anti-AF4 antibody A302-344A, which recognizes an upstream epitope. For blotting immunoprecipitated AF4, A302-344A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A302-345A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 175 and 225 of human proto-oncogene AF4.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Proto-oncogene AF-4 (or FEL) is a product of a chromosomal aberration of the human gene AFF1, which is associated with acute leukemias. The fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in a MLL-AF-4 chimeric transcription factor in which AF-4 contributes transcriptional effector properties and requires cell-specific accessory factors. MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia. The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AF4/FMR2 family member 1; AF4/FMR2 family, member 1; ALL1-fused gene from chromosome 4 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2; pre-B-cell monocytic leukemia partner 1; Protein AF-4; Protein FEL; Proto-oncogene AF4
Gene Aliases: AF4; AFF1; FEL; MLLT2; PBM1
UniProt ID: (Human) P51825
Entrez Gene ID: (Human) 4299
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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