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            • Primary Antibodies ›
            • AIP Antibodies

            Invitrogen

            AIP Monoclonal Antibody (10G8)

            View all (13) AIP antibodies
            Datasheet
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            Catalog # MA5-49238
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            Price (EUR)
            415,00
            100 µg
             
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            Cite AIP Monoclonal Antibody (10G8)

            • Antibody Testing Data (2)
            Application
            AIP Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            AIP Antibody (MA5-49238) in Flow

            Flow cytometry analysis of AIP in A549 cells using AIP Monoclonal Antibody (10G8) (Product # MA5-49238), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control anti... View More
            View Product

            AIP Antibody in Flow Cytometry (Flow)
            AIP Antibody in Flow Cytometry (Flow)

            Product Details

            MA5-49238

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            0.1-0.5 µg/mL
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            0.5-1 µg/mL
            -

            Immunocytochemistry (ICC/IF)

            2 µg/mL
            -

            Flow Cytometry (Flow)

            1-3 µg/1x10^6 cells
            -
            Product Specifications

            Species Reactivity

            Human, Mouse, Non-human primate, Rat

            Host/Isotype

            Mouse / IgG2b

            Class

            Monoclonal

            Type

            Antibody

            Clone

            10G8

            Immunogen

            E.coli-derived human ARA9 recombinant protein (Position: D91-H330).
            View immunogen

            Conjugate

            Unconjugated

            Form

            Lyophilized

            Concentration

            500 µg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS with 4mg trehalose

            Contains

            0.05mg sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_3074896

            Product Specific Information

            Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

            Human ARA9 shares 95% amino acid (aa) sequence identity with both mouse and rat ARA9.

            Positive Control - WB: human HELA whole cell, human HEPG2 whole cell, human placenta tissue, human CACO-2 whole cell, human U20S whole cell, monkey COS-7 whole cell, human K562 whole cell, rat heart tissue, rat skeletal muscle tissue, rat brain tissue, rat spleen tissue, mouse brain tissue, mouse HEPA1-6 whole cell. IHC: human intestinal cancer tissue, human tonsil tissue, rat spleen tissue. ICC/IF: A431 cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

            Target Information

            The protein encoded by this gene is a receptor for aryl hydrocarbons and a ligand-activated transcription factor. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. This protein can regulate the expression of many xenobiotic metabolizing enzymes. Also, the encoded protein can bind specifically to and inhibit the activity of hepatitis B virus.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: AH receptor-interacting protein; AIP; Aryl-hydrocarbon receptor-interacting protein; HBV X-associated protein 2; HBV-X associated protein; Immunophilin homolog ARA9; Immunophilin XAP2; XAP-2

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            Gene Aliases: AA408703; AIP; ARA9; AW476050; D19Bwg1412e; FKBP16; FKBP37; SMTPHN; XAP-2; XAP2

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            UniProt ID: (Human) O00170, (Mouse) O08915, (Rat) Q5FWY5

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            Entrez Gene ID: (Human) 9049, (Mouse) 11632, (Rat) 282827

            View more View less

            Function(s)

            transcription coactivator activity signal transducer activity protein binding transcription factor binding aryl hydrocarbon receptor binding GAF domain binding unfolded protein binding transcription cofactor activity chaperone

            Process(es)

            protein targeting to mitochondrion xenobiotic metabolic process signal transduction regulation of protein kinase A signaling protein maturation by protein folding negative regulation of cyclic-nucleotide phosphodiesterase activity regulation of nucleic acid-templated transcription protein folding
            It has to be done as per old AB suggested Products section.
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