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AIP Antibody (MA5-49238) in Flow
Flow cytometry analysis of AIP in A549 cells using AIP Monoclonal Antibody (10G8) (Product # MA5-49238), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control anti... View More
Product Details
MA5-49238
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
10G8
Immunogen
E.coli-derived human ARA9 recombinant protein (Position: D91-H330).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074896
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human ARA9 shares 95% amino acid (aa) sequence identity with both mouse and rat ARA9.
Positive Control - WB: human HELA whole cell, human HEPG2 whole cell, human placenta tissue, human CACO-2 whole cell, human U20S whole cell, monkey COS-7 whole cell, human K562 whole cell, rat heart tissue, rat skeletal muscle tissue, rat brain tissue, rat spleen tissue, mouse brain tissue, mouse HEPA1-6 whole cell. IHC: human intestinal cancer tissue, human tonsil tissue, rat spleen tissue. ICC/IF: A431 cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is a receptor for aryl hydrocarbons and a ligand-activated transcription factor. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. This protein can regulate the expression of many xenobiotic metabolizing enzymes. Also, the encoded protein can bind specifically to and inhibit the activity of hepatitis B virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AH receptor-interacting protein; AIP; Aryl-hydrocarbon receptor-interacting protein; HBV X-associated protein 2; HBV-X associated protein; Immunophilin homolog ARA9; Immunophilin XAP2; XAP-2
Gene Aliases: AA408703; AIP; ARA9; AW476050; D19Bwg1412e; FKBP16; FKBP37; SMTPHN; XAP-2; XAP2
UniProt ID: (Human) O00170, (Mouse) O08915, (Rat) Q5FWY5
Entrez Gene ID: (Human) 9049, (Mouse) 11632, (Rat) 282827
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