Proteintech

AKAP3 Polyclonal Antibody

AKAP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AKAP3 Antibody (13907-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using (Product # 13907-1-AP) (AKAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

13907-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AKAP3 Fusion Protein Ag4885 (1-300 aa encoded by BC047535)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.49 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSEKVDWLQ SQNGVCKVDV YSPGDNQAQD WKMDTSTDPV RVLSWLRRDL EKSTAEFQDV RFKPGESFGG ETSNSGDPHK GFSVDYYNTT TKGTPERLHF EMTHKEIPCQ GPRAQLGNES SVDEVSFYAN RLTNLVIAMA RKEINEKIDG SENKCVYQSL YMGNEPTPTK SLSKIASELV NETVSACSRN AAPDKAPGSG DRVSGSSQSP PNLKYKSTLK IKESTKERQG PDDKPPSKKS FFYKEVFESR NGDYAREGGR FFPRERKRFR GQERPDDFTA SVSEGIMTYA NSVVSDMMVS I (1-300 aa encoded by BC047535)

Target Information

The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins, designated A-kinase anchoring proteins (AKAP). AKAP 3, also known as AKAP 110, FSP95, PRKA3 and SOB1, binds both PKA and PDE4A and functions as a scaffolding protein in spermatozoa to regulate local cAMP concentrations and modulate sperm functions. Expression of AKAP 3 in normal tissues is restricted to the testis, where bicarbonate stimulates tyrosine phosphorylation of AKAP 3, thereby increasing its recruitment of PKA. AKAP-3 also exhibits high expression in patients with epithelial ovarian cancer (EOC). It demonstrates tumor-restricted expression and appears to be associated with worse overall survival, which make AKAP 3 a potential target for antigen-specific immunotherapy in EOC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Homozygous variants in AKAP3 induce asthenoteratozoospermia and male infertility.

Journal of medical genetics

Liu C,Shen Y,Tang S,Wang J,Zhou Y,Tian S,Wu H,Cong J,He X,Jin L,Cao Y,Yang Y,Zhang F

Wed Feb 01 00:00:00 EST 2023

Homozygous variants in AKAP3 induce asthenoteratozoospermia and male infertility.

Journal of medical genetics

Liu C,Shen Y,Tang S,Wang J,Zhou Y,Tian S,Wu H,Cong J,He X,Jin L,Cao Y,Yang Y,Zhang F

Wed Feb 01 00:00:00 EST 2023

STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis.

Molecular & cellular proteomics : MCP

Yu W,Li Y,Chen H,Cui Y,Situ C,Yao L,Zhang X,Lu S,Liu L,Li L,Ren J,Guo Y,Huo Z,Chen Y,Li H,Jiang T,Gu Y,Wang C,Zhu T,Li Y,Hu Z,Guo X

Thu Jun 01 00:00:00 EDT 2023

STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis.

Molecular & cellular proteomics : MCP

Yu W,Li Y,Chen H,Cui Y,Situ C,Yao L,Zhang X,Lu S,Liu L,Li L,Ren J,Guo Y,Huo Z,Chen Y,Li H,Jiang T,Gu Y,Wang C,Zhu T,Li Y,Hu Z,Guo X

Thu Jun 01 00:00:00 EDT 2023

The cancer-testis antigen a-kinase anchor protein 3 facilitates breast cancer progression via activation of the PTEN/PI3K/AKT/mTOR signaling.

Bioengineered

Zhan CH,Ding DS,Zhang W,Wang HL,Mao ZY,Liu GJ

Fri Apr 01 00:00:00 EDT 2022

IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice.

Developmental biology

Kaneda Y,Miyata H,Shimada K,Oyama Y,Iida-Norita R,Ikawa M

Mon Aug 01 00:00:00 EDT 2022

Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.

Frontiers in cell and developmental biology

Xu Y,Han Q,Ma C,Wang Y,Zhang P,Li C,Cheng X,Xu H

Tue Aug 03 00:00:00 EDT 2021

SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

Proceedings of the National Academy of Sciences of the United States of America

Miyata H,Oura S,Morohoshi A,Shimada K,Mashiko D,Oyama Y,Kaneda Y,Matsumura T,Abbasi F,Ikawa M

Tue Aug 31 00:00:00 EDT 2021

The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development.

Development (Cambridge, England)

Beckers A,Adis C,Schuster-Gossler K,Tveriakhina L,Ott T,Fuhl F,Hegermann J,Boldt K,Serth K,Rachev E,Alten L,Kremmer E,Ueffing M,Blum M,Gossler A

Mon Jun 15 00:00:00 EDT 2020

CFAP43 modulates ciliary beating in mouse and Xenopus.

Developmental biology

Rachev E,Schuster-Gossler K,Fuhl F,Ott T,Tveriakhina L,Beckers A,Hegermann J,Boldt K,Mai M,Kremmer E,Ueffing M,Blum M,Gossler A

Sun Mar 15 00:00:00 EDT 2020

Fluoride exposure alters the ultra-structure of sperm flagellum via reducing key protein expressions in testis.

Chemosphere

Liang C,He Y,Liu Y,Gao Y,Han Y,Li X,Zhao Y,Wang J,Zhang J

Fri May 01 00:00:00 EDT 2020

Biallelic mutations in PMFBP1 cause acephalic spermatozoa.

Clinical genetics

Sha YW,Wang X,Xu X,Ding L,Liu WS,Li P,Su ZY,Chen J,Mei LB,Zheng LK,Wang HL,Kong SB,You M,Wu JF

Fri Feb 01 00:00:00 EST 2019

Biallelic mutations in PMFBP1 cause acephalic spermatozoa.

Clinical genetics

Sha YW,Wang X,Xu X,Ding L,Liu WS,Li P,Su ZY,Chen J,Mei LB,Zheng LK,Wang HL,Kong SB,You M,Wu JF

Fri Feb 01 00:00:00 EST 2019

Biallelic mutations in PMFBP1 cause acephalic spermatozoa.

Clinical genetics

Sha YW,Wang X,Xu X,Ding L,Liu WS,Li P,Su ZY,Chen J,Mei LB,Zheng LK,Wang HL,Kong SB,You M,Wu JF

Fri Feb 01 00:00:00 EST 2019

Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.

Nature communications

Shen Y,Zhang F,Li F,Jiang X,Yang Y,Li X,Li W,Wang X,Cheng J,Liu M,Zhang X,Yuan G,Pei X,Cai K,Hu F,Sun J,Yan L,Tang L,Jiang C,Tu W,Xu J,Wu H,Kong W,Li S,Wang K,Sheng K,Zhao X,Yue H,Yang X,Xu W

Fri Jan 25 00:00:00 EST 2019

FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.

Proceedings of the National Academy of Sciences of the United States of America

Liu T,Zhang H,Sun L,Zhao D,Liu P,Yan M,Zaidi N,Izadmehr S,Gupta A,Abu-Amer W,Luo M,Yang J,Ou X,Wang Y,Bai X,Wang Y,New MI,Zaidi M,Yuen T,Liu C

Tue Jul 18 00:00:00 EDT 2017

FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.

Proceedings of the National Academy of Sciences of the United States of America

Liu T,Zhang H,Sun L,Zhao D,Liu P,Yan M,Zaidi N,Izadmehr S,Gupta A,Abu-Amer W,Luo M,Yang J,Ou X,Wang Y,Bai X,Wang Y,New MI,Zaidi M,Yuen T,Liu C

Tue Jul 18 00:00:00 EDT 2017

CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.

Journal of cell science

Young SA,Miyata H,Satouh Y,Aitken RJ,Baker MA,Ikawa M

Thu Dec 01 00:00:00 EST 2016

CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.

Journal of cell science

Young SA,Miyata H,Satouh Y,Aitken RJ,Baker MA,Ikawa M

Thu Dec 01 00:00:00 EST 2016

Interactome of the negative regulator of nuclear import BRCA1-binding protein 2.

Scientific reports