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            • Primary Antibodies ›
            • AKT1 Antibodies

            OriGene

            AKT1 Monoclonal Antibody (OTI4C11), TrueMAB™

            Advanced Verification
            View all (194) AKT1 antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # TA504235
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            Price (USD)
            540.00
             
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            Datasheet
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            Cite AKT1 Monoclonal Antibody (OTI4C11), TrueMAB™

            • Antibody Testing Data (10)
            • Advanced Verification (1)
            Application
            AKT1 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 11

            AKT1 Antibody (TA504235) in ICC/IF

            Anti-AKT1 mouse monoclonal antibody (TA504235) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1(RC220257).
            View Product

            AKT1 Antibody in Immunocytochemistry (ICC/IF)
            AKT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            AKT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            AKT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            AKT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            AKT1 Antibody in Western Blot (WB)
            AKT1 Antibody in Western Blot (WB)
            AKT1 Antibody in Western Blot (WB)
            AKT1 Antibody in Flow Cytometry (Flow)
            AKT1 Antibody in Flow Cytometry (Flow)
            AKT1 Antibody

            Product Details

            TA504235

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:2,000
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1:150
            -

            Immunocytochemistry (ICC/IF)

            1:100
            -

            Flow Cytometry (Flow)

            1:100
            -
            Product Specifications

            Species Reactivity

            Dog, Human, Mouse, Rat

            Host/Isotype

            Mouse / IgG1

            Class

            Monoclonal

            Type

            Antibody

            Clone

            OTI4C11

            Immunogen

            Full length human recombinant protein of human AKT1 produced in HEK293T cell.
            View immunogen

            Conjugate

            Unconjugated
            • Biotin 
            • Request custom conjugation

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS with 1% BSA, 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Target Information

            AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; murine thymoma viral (v-akt) oncogene homolog 1; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

            View more View less

            Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

            View more View less

            UniProt ID: (Human) P31749, (Mouse) P31750, (Rat) P47196

            View more View less

            Entrez Gene ID: (Dog) 490878, (Human) 207, (Mouse) 11651, (Rat) 24185

            View more View less

            Function(s)

            protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase

            Process(es)

            protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process translation regulation of translation cellular protein modification process protein phosphorylation negative regulation of protein kinase activity nitric oxide biosynthetic process activation-induced cell death of T cells inflammatory response cellular response to DNA damage stimulus response to oxidative stress signal transduction G-protein coupled receptor signaling pathway germ cell development aging cell proliferation insulin receptor signaling pathway apoptotic mitochondrial changes response to heat negative regulation of autophagy negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process negative regulation of endopeptidase activity regulation of neuron projection development regulation of phosphatidylinositol 3-kinase signaling glucose transport phosphorylation protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development cell projection organization cell differentiation protein catabolic process platelet activation hyaluronan metabolic process positive regulation of cell growth regulation of cell migration endocrine pancreas development T cell costimulation regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food positive regulation of cellular protein metabolic process peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus ERBB2 signaling pathway glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process positive regulation of epidermal growth factor receptor signaling pathway negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import negative regulation of JNK cascade protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling regulation of nitric-oxide synthase activity positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation response to growth hormone mammary gland epithelial cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to organic cyclic compound cellular response to hypoxia establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis chemical synaptic transmission, postsynaptic negative regulation of protein kinase activity by protein phosphorylation positive regulation of protein localization to nucleus negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of cell cycle checkpoint negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand carbohydrate metabolic process glycogen metabolic process transport apoptotic process cytoskeleton organization multicellular organism development nervous system development carbohydrate transport response to hormone regulation of protein localization cellular response to decreased oxygen levels anagen cellular response to growth factor stimulus cellular response to peptide
            It has to be done as per old AB suggested Products section.
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