Loading ...

Invitrogen

ALOX15 Polyclonal Antibody

2 Published Figures

2 References

View all (21) ALOX15 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (2)

Application

ALOX15 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

ALOX15 Antibody (PA5-15065) in ICC/IF

Immunocytochemistry analysis of ALOX15 in HeLa cells. Samples were incubated in ALOX15 polyclonal antibody (Product # PA5-15065) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

ALOX15 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALOX15 Antibody in Immunohistochemistry (IHC)

ALOX15 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Product Details

PA5-15065

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

1:10-1:50

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2227014

Target Information

ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pro-ferroptotic signaling promotes arterial aging via vascular smooth muscle cell senescence.

Nature communications

Sun DY,Wu WB,Wu JJ,Shi Y,Xu JJ,Ouyang SX,Chi C,Shi Y,Ji QX,Miao JH,Fu JT,Tong J,Zhang PP,Zhang JB,Li ZY,Qu LF,Shen FM,Li DJ,Wang P

Published figure using ALOX15 polyclonal antibody (Product # PA5-15065) in Immunohistochemistry

Fri Feb 16 00:00:00 UTC 2024

Pro-ferroptotic signaling promotes arterial aging via vascular smooth muscle cell senescence.

Nature communications

Sun DY,Wu WB,Wu JJ,Shi Y,Xu JJ,Ouyang SX,Chi C,Shi Y,Ji QX,Miao JH,Fu JT,Tong J,Zhang PP,Zhang JB,Li ZY,Qu LF,Shen FM,Li DJ,Wang P

Published figure using ALOX15 polyclonal antibody (Product # PA5-15065) in Immunohistochemistry

Fri Feb 16 00:00:00 UTC 2024

Bioinformatics

Protein Aliases: 12-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; 15-LOX; 15-LOX2; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate 15-lipoxygenase; Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; Polyunsaturated fatty acid lipoxygenase ALOX15

Gene Aliases: 12-LOX; 15-LOX-1; 15LOX-1; ALOX15; LOG15

UniProt ID: (Human) P16050

Entrez Gene ID: (Human) 246

Function(s)

arachidonate 12-lipoxygenase activity iron ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding hepoxilin-epoxide hydrolase activity arachidonate 15-lipoxygenase activity hepoxilin A3 synthase activity eoxin A4 synthase activity oxygenase

Process(es)

ossification negative regulation of adaptive immune response phosphatidylethanolamine biosynthetic process leukotriene metabolic process inflammatory response positive regulation of cell-substrate adhesion arachidonic acid metabolic process lipoxygenase pathway bone mineralization positive regulation of actin filament polymerization positive regulation of heterotypic cell-cell adhesion response to endoplasmic reticulum stress regulation of peroxisome proliferator activated receptor signaling pathway cellular response to interleukin-13 wound healing apoptotic cell clearance hepoxilin biosynthetic process oxidation-reduction process positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion regulation of engulfment of apoptotic cell lipoxin A4 biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more