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Active Motif
AM-Tag Recombinant Mouse Monoclonal Antibody, AbFlex®
Application
FIGURE: 1 / 2
AM-Tag Antibody (91111) in WB
AbFlex™ AM-Tag antibody (rAb) tested by Western Blot. Active Motif’s pAM_1C_JunD Vector (Catalog No. 53044) was transfected, mock transfected or transfected with pAM_1C Empty Vector (Catalog No. 53023) into HeLa or HCT116 cells using 10 µg DNA and 30 µl FuGENE transfection reagent, 48 hours post-transfection nuclear lysates were prepared. 20 µg lysate was loaded per well. Western blot was performed using a 1:500 dilution of AM-Tag antibody.
Product Details
91111
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG2a
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Immunogen
This antibody was raised against Active Motif's unique AM-Tag sequence.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
140mM HEPES/32mM sodium acetate, pH 7.5, with 30% glycerol, 70mM NaCl
Contains
0.035% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2793779
Target Information
The AM-Tag antibody is specifically designed for use with Active Motif's unique AM-Tag sequence in combination with the Tag-ChIP-IT® Kit (Catalog No. 53022). Simply clone your protein of interest into Active Motif's pAM_1C Empty Vector (Catalog No. 53023), or add the AM-Tag sequence to the C-terminus of your protein expression vector. Following transfection, cell lysates are prepared and screened by Western blot for expression of the AM-Tag fusion protein using the AM-Tag antibody. Optimization may be required to identify the optimal conditions for transfection and protein expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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