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Invitrogen
AMPK beta-1 Recombinant Rabbit Monoclonal Antibody (7G8U1)
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Application
FIGURE: 1 / 6
AMPK beta-1 Antibody (MA5-35486) in ICC/IF
Confocal imaging (Immunocytochemistry) of AMPK beta-1 in NIH/3T3 cells. Samples were incubated with AMPK beta-1 Monoclonal antibody (Product # MA5-35486) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Product Details
MA5-35486
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7G8U1
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human AMPKbeta1 (Q9Y478)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849387
Product Specific Information
Immunogen sequence: EEIKAPEKEE FLAWQHDLEV NDKAPAQARP TVFRWTGGGK EVYLSGSFNN WSKLPLTRSH NNFVAILDLP EGEHQYKFFV DGQWTHDPSE PIVTSQLGTV N
Target Information
AMPK beta 1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-AMP-activated protein kinase 40 kDa subunit; 5'-AMP-activated protein kinase beta-1 subunit; 5'-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase, beta subunit; 5-AMP-activated protein kinase beta subunit; AAKB1; AMP-activated protein kinase beta subunit; AMPK beta -1 chain; AMPK beta 1; AMPK beta-1 chain; AMPK subunit beta-1; AMPK-beta1; AMPKb; kinase AMPK-beta1; kinase pAMPK; pAMPK; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1
Gene Aliases: 1300015D22Rik; AMPK; AU021155; E430008F22; HAMPKb; PRKAB1
UniProt ID: (Human) Q9Y478, (Mouse) Q9R078, (Rat) P80386
Entrez Gene ID: (Human) 5564, (Mouse) 19079, (Rat) 83803
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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