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Application
FIGURE: 1 / 1
AMPK beta-2 Antibody (711321) in WB
Western blot analysis was performed on Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2). The blots were probed with Anti-AMPK-b2 Recombinant Rabbit Superclonal™ Antibody (Product # 711321, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 30 kDa band corresponding to AMPK-b2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Nov... View More
Product Details
711321
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
6HCLC
Immunogen
Peptides corresponding to Human PRKAB2 (aa 167-180, 26-33)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633161
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Horse and Cat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
AMPK Beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-AMP-activated protein kinase subunit beta-2; 5'-AMP-activated protein kinase, beta-2 subunit; AMP-activated protein kinase beta 2 non-catalytic subunit; AMP-activated protein kinase beta-2 regulatory subunit; AMPK beta 2; AMPK beta-2 chain; AMPK subunit beta-2; AMPKbeta2; protein kinase, AMP-activated, beta 2 non-catalytic subunit
Gene Aliases: 5730553K21Rik; AW049591; BB124140; PRKAB2
UniProt ID: (Human) O43741, (Rat) Q9QZH4, (Mouse) Q6PAM0
Entrez Gene ID: (Human) 5565, (Rat) 64562, (Mouse) 108097
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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