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Application
FIGURE: 1 / 1
ANKRD28 Antibody (PA5-109392) in WB
Western blot analysis of ANKRD28 in extracts of various cell lines. Samples were incubated with ANKRD28 Polyclonal antibody (Product # PA5-109392) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-109392
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ANKRD28 (NP_056014.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.58 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854803
Product Specific Information
Immunogen sequence: MAFLKLRDQP SLVQAIFNGD PDEVRALIFK KEDVNFQDNE KRTPLHAAAY LGDAEIIELL ILSGARVNAK DSKWLTPLHR AVASCSEEAV QVLLKHSADV NARDKNWQTP LHIAAANKAV KCAEALVPLL SNVNVSDRAG RTALHHAAFS GHGEMVKLLL SRGANINAFD KKDRRAIHWA AYMGHIEVVK LLVSHGAEVT CKDKKSYTPL HAAASSGMIS VVKYLLDLGV DMNEPNAYGN TPLHVACYNG QDVVVNELID CGAIVNQKNE KGFTPLHFAA ASTHGALCLE LLVGNGADVN
Target Information
Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Selectively inhibits the phosphatase activity of PPP1C. Targets PPP1C to modulate HNRPK phosphorylation. ANKRD28 selectively regulated phosphorylation of hnRNP-K at ser284, an effect that was enhanced in vivo by an S1013A/S1017A double mutant, suggesting that ANKRD28 targets PP1 in a regulated manner to dephosphorylate hnRNP-K selectively at ser284.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ankyrin repeat domain-containing protein 28; phosphatase interactor targeting K protein; Phosphatase interactor targeting protein hnRNP K; PITK; PP6-ARS-A; protein phosphatase 1, regulatory subunit 65; protein phosphatase 6 ankyrin repeat subunit A; Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; serine/threonine-protein phosphatase 6 regulatory subunit ARS-A
Gene Aliases: AI465466; AI604979; ANKRD28; E430019N21Rik; KIAA0379; mKIAA0379; PITK; PPP1R65
UniProt ID: (Human) O15084, (Mouse) Q505D1
Entrez Gene ID: (Human) 23243, (Mouse) 105522
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