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Bethyl Laboratories

AP4 Polyclonal Antibody

View all (17) TFAP4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Application

AP4 Antibody in Immunoprecipitation (IP)

FIGURE: 1 / 1

AP4 Antibody (A302-760A) in IP

Detection of human AP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AP4 antibody (Product # A302-760A) used for IP at 3 µg/mg lysate. AP4 was also immunoprecipitated by rabbit anti-AP4 antibody A302-761A, which recognizes a downstream epitope. For blotting immunoprecipitated AP4, A302-761A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Product Details

A302-760A

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 50 and 100 of human Activating Enhancer-Binding Protein 4.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Transcription factors of the basic helix-loop-helix-zipper(bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating enhancer-binding protein 4; bHLHc41; Class C basic helix-loop-helix protein 41; Transcription factor AP-4; transcription factor AP-4 (activating enhancer-binding protein 4)

Gene Aliases: AP-4; BHLHC41; TFAP4

UniProt ID: (Human) Q01664

Entrez Gene ID: (Human) 7023

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity E-box binding DNA-binding transcription factor

Process(es)

transcription from RNA polymerase II promoter protein complex assembly DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator negative regulation of cell proliferation positive regulation of apoptotic process negative regulation of DNA binding negative regulation by host of viral transcription positive regulation by host of viral transcription negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of cell cycle arrest cellular response to dexamethasone stimulus positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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