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Bethyl Laboratories
APC4 Polyclonal Antibody
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Application
FIGURE: 1 / 1
APC4 Antibody (A301-175A) in IP
Detection of human APC4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC4 antibody (Product # A301-175A) used for IP at 3 µg/mg lysate. APC4 was also immunoprecipitated by rabbit anti-APC4 antibody A301-176A, which recognizes a downstream epitope. For blotting immunoprecipitated APC4, A301-176A was used at 1.0 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
Product Details
A301-175A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 425 and 475 of human anaphase-promoting complex subunit 4.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan
Target Information
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Anaphase-promoting complex subunit 4; APC4; Cyclosome subunit 4
Gene Aliases: ANAPC4; APC4
UniProt ID: (Human) Q9UJX5
Entrez Gene ID: (Human) 29945
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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