Loading ...

Invitrogen

APOE Polyclonal Antibody

Advanced Verification

2 Published Figures

4 References

View all (61) APOE antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

APOE Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

APOE Antibody (PA5-27088) in ICC/IF

Immunofluorescence analysis of APOE was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with APOE Rabbit Polyclonal Antibody (Product # PA5-27088) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilut... View More

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APOE Antibody in Immunoprecipitation (IP)

APOE Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

APOE Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates tested owing to their inherent genetic constitution. Relative expression of APOE was observed in Hep G2 and Caco-2 as compared to A-431 and Daudi using Anti-APOE Monoclonal Antibody (E6D7) (Product # PA5-27088) in Western Blot. {RE}

Product Details

PA5-27088

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

ELISA (ELISA)

1:1,000-1:10,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.66 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544564

Product Specific Information

Recommended positive controls: HepG2, Conditional medium from differentiated human primary preadipocyte, human plasma.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The P522R protective variant of PLCG2 promotes the expression of antigen presentation genes by human microglia in an Alzheimer's disease mouse model.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Claes C,England WE,Danhash EP,Kiani Shabestari S,Jairaman A,Chadarevian JP,Hasselmann J,Tsai AP,Coburn MA,Sanchez J,Lim TE,Hidalgo JLS,Tu C,Cahalan MD,Lamb BT,Landreth GE,Spitale RC,Blurton-Jones M,Davtyan H

PA5-27088 was used in Immunohistochemistry (Frozen) to provide the first evidence that the PLCG2-P522R variant increases the capacity of microglia to recruit T cells and present antigens, promoting a microglial transcriptional state that has recently been shown to be reduced in AD patient brains.

Sat Oct 01 00:00:00 UTC 2022

Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease.

Molecular neurodegeneration

Claes C,Danhash EP,Hasselmann J,Chadarevian JP,Shabestari SK,England WE,Lim TE,Hidalgo JLS,Spitale RC,Davtyan H,Blurton-Jones M

Published figure using APOE polyclonal antibody (Product # PA5-27088) in Immunohistochemistry (Frozen)

Fri Jul 23 00:00:00 UTC 2021

Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease.

Molecular neurodegeneration

Claes C,Danhash EP,Hasselmann J,Chadarevian JP,Shabestari SK,England WE,Lim TE,Hidalgo JLS,Spitale RC,Davtyan H,Blurton-Jones M

Published figure using APOE polyclonal antibody (Product # PA5-27088) in Immunohistochemistry (Frozen)

Fri Jul 23 00:00:00 UTC 2021

Morphological analysis of Apolipoprotein E binding to Aβ Amyloid using a combination of Surface Plasmon Resonance, Immunogold Labeling and Scanning Electron Microscopy.

BMC biotechnology

Islam T,Gharibyan AL,Lee CC,Olofsson A

Published figure using APOE polyclonal antibody (Product # PA5-27088) in Immunocytochemistry

Wed Dec 11 00:00:00 UTC 2019

Complement-Mediated Regulation of Apolipoprotein E in Cultured Human RPE Cells.

Investigative ophthalmology & visual science

Yang P,Skiba NP,Tewkesbury GM,Treboschi VM,Baciu P,Jaffe GJ

PA5-27088 was used in Immunocytochemistry to study the effect of complement activation on induction of apolipoprotein E accumulation in human retinal pigment epithelial cells.

Thu Jun 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Apo-E; ApoE4; Apolipoprotein; Apolipoprotein E; apolipoprotein E3; apolipoprotein E4; Apoprotein; B2G1; BG; MGC1571

Gene Aliases: AD2; APO-E; APOE; LDLCQ5; LPG

UniProt ID: (Human) P02649

Entrez Gene ID: (Human) 348

Function(s)

beta-amyloid binding lipid transporter activity protein binding phospholipid binding heparin binding lipid binding cholesterol binding antioxidant activity cholesterol transporter activity identical protein binding protein homodimerization activity metal chelating activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding apolipoprotein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

APOE Polyclonal Antibody

APOE Antibody (PA5-27088) in ICC/IF

Product Details

PA5-27088

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!

APOE Polyclonal Antibody

APOE Antibody (PA5-27088) in ICC/IF

Product Details

PA5-27088

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Related Products for

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!