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Application
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APS Antibody (PA5-62326) in ICC/IF
Immunofluorecent analysis of APS in human cell line MCF7 using APS Polyclonal Antibody (Product # PA5-62326). Staining shows localization to cytosol.
Product Details
PA5-62326
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human APS. Recombinant protein control fragment (Product #RP-99507).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2638097
Product Specific Information
Immunogen sequence: RDKWTRRLRL SRTLAAKVEL VDIQREGALR FMVADD
Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.
Target Information
APS (adapter protein with Pleckstrin homology and Src homology 2 domains) a member of the Lnk family, is an adaptor protein that is involved in B-cell signaling, insulin signaling, cytoskeletal reorganization. It is tyrosine phosphorylated by JAK2, KIT and other kinases during B-cell receptor stimulation of many different cytokines, chemokines and leukokines. APS has been shown to inhibit the JAK-STAT pathway in collaboration with c-Cbl. It is located in the cytoplasm in pre-PDGF (platelet derived growth factor) stimulated cells and localizes to the plasma membrane and peripheral regions upon PDGF stimulation. This protein is expressed in B-cells and mast cells when detected in haematopoietic cell lines yet is also expressed in many different tissues including spleen, prostrate, testis, uterus, small intestine and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adapter protein with pleckstrin homology and Src homology 2 domains; Alpha-1-antitrypsin; SH2 and PH domain-containing adapter protein APS; SH2B adapter protein 2
Gene Aliases: APS; SH2B2
UniProt ID: (Human) O14492
Entrez Gene ID: (Human) 10603
Performance Guarantee
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