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            • Primary Antibodies ›
            • ARF5 Antibodies

            Invitrogen

            ARF5 Polyclonal Antibody

            View all (8) ARF5 antibodies
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            Cite ARF5 Polyclonal Antibody

            • Antibody Testing Data (3)
            Application
            ARF5 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            ARF5 Antibody (PA5-21608) in ICC/IF

            Immunocytochemistry-Immunofluorescence analysis of ARF5 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ARF5 Polyclonal Antibody (Product # PA5-21608) diluted at 1:500. Blue: Hoechst 33342 staining.
            View Product

            ARF5 Antibody in Immunocytochemistry (ICC/IF)
            ARF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            ARF5 Antibody in Western Blot (WB)

            Product Details

            PA5-21608

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:3,000
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1:100-1:1,000
            -

            Immunocytochemistry (ICC/IF)

            1:100-1:1,000
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF5. The exact sequence is proprietary.

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.67 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7, with 20% glycerol

            Contains

            0.025% ProClin 300

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_11152723

            Product Specific Information

            Recommended positive controls: 293T, A431, HeLa, HepG2.

            Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Sheep (100%), Bovine (100%).

            Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

            Target Information

            ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: ADP-ribosylation factor 5; EFL2; EPLG3; LERK3

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            Gene Aliases: ARF5

            View more View less

            UniProt ID: (Human) P84085

            View more View less

            Entrez Gene ID: (Human) 381

            View more View less

            Function(s)

            GTPase activity protein binding GTP binding G-protein

            Process(es)

            retrograde vesicle-mediated transport, Golgi to ER small GTPase mediated signal transduction protein transport
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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