Invitrogen

ARF5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (11) ARF5 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

ARF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

ARF5 Antibody (PA5-31432) in IHC (P)

Immunohistochemistry (Paraffin) analysis of ARF5 was performed in paraffin-embedded mouse lung tissue using ARF5 Polyclonal Antibody (Product # PA5-31432) at a dilution of 1:500.

Product Image Gallery

ARF5 Antibody — Fig 6 Arf6 is recruited by R. typhi RalF and is required for infection. (A) Ectopically expressed RalFRtFL co-localizes with Arf6 but not Arf5. HeLa cells co-expressing EYFP, EYFP-RalFRtFL or EYFP-RalFRbFL and mRFP-Arf6 (left) or -Arf5 (right) were fixed with 4% para-formaldehyde. Nuclei were stained with DAPI (blue). (Scale bar: 10 µm). (B) RalFRtFL pull-down of Arf6. Lysates from HEK293T cells expressing mRFP-Arf5 or -Arf6 were incubated with HisPur Cobalt resin bound with rHis-RalFRtFL or resin alone. Bound proteins were eluted with imidazole and analyzed by protein immunoblot using antibodies as indicated. (C) Arf6 is recruited during R. typhi entry. HeLa cells expressing mRFP-Arf5 or -Arf6 (red) were infected with partially purified R. typhi (MOI ~100). Ten minutes post infection, cells were fixed and R. typhi detected with anti-R. typhi serum (green). DAPI (blue) is shown in the merged image. Boxed regions are enlarged to show detail. White arrowheads indicate R. typhi. (Scale bar: 5 µm). (D) Arf6 knockdown inhibits R. typhi infection. HeLa cells transfected with negative, Arf6, or Arf5 siRNA were infected with partially purified R. typhi (MOI ~100). At 2 hrs post infection, cells were fixed, plasma membrane stained with Alexa Fluor 594 wheat germ agglutinin, and R. typhi detected with rat anti-R. typhi serum and Alexa Fluor 488 anti-rat antibody. The number of R. typhi per host cell was counted for 100 host cells for three independent experiments. Error bars represent mean ± SD (Student’s two-sided t-test). (Scale bar: 5µm). (E) Confirmat {KD}

Product Details

PA5-31432

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 1 and 161 of Human ARF5

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2548906

Product Specific Information

Recommended positive controls: 293T, Raji, mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (85%), Pig (100%), Chicken (98%), Sheep (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion.

PLoS pathogens

Rennoll-Bankert KE,Rahman MS,Gillespie JJ,Guillotte ML,Kaur SJ,Lehman SS,Beier-Sexton M,Azad AF

PA5-31432 was used in western blot to study different bacterial guanine nucleotide exchange factor of ADP-ribosylation factors

Sat Aug 01 00:00:00 EDT 2015

Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion.

PLoS pathogens

Rennoll-Bankert KE,Rahman MS,Gillespie JJ,Guillotte ML,Kaur SJ,Lehman SS,Beier-Sexton M,Azad AF

PA5-31432 was used in western blot to study different bacterial guanine nucleotide exchange factor of ADP-ribosylation factors

Sat Aug 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: ADP-ribosylation factor 5; EFL2; EPLG3; LERK3

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Gene Aliases: ARF5

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UniProt ID: (Human) P84085, (Mouse) P84084

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Entrez Gene ID: (Human) 381, (Mouse) 11844

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Function(s)

GTPase activity protein binding GTP binding nucleotide binding G-protein

Process(es)

retrograde vesicle-mediated transport, Golgi to ER small GTPase mediated signal transduction protein transport transport vesicle-mediated transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ARF5 Polyclonal Antibody

ARF5 Antibody (PA5-31432) in IHC (P)

Product Details

PA5-31432

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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