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Invitrogen
ARSA Polyclonal Antibody
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Application
FIGURE: 1 / 6
ARSA Antibody (PA5-78824) in ICC/IF
Immunocytochemistry analysis of ARSA using anti-ARSA antibody (Product # PA5-78824) . ARSA was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-ARSA antibody (Product # PA5-78824) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-78824
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA (454-482aa QALKQLQLLKAQLDAAVTFGPSQVARGED).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745940
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Testis Tissue, Rat Pancreas Tissue, Rat Skeletal Muscle Tissue, Mouse Kidney Tissue, MCF-7 whole cell. IHC: Rat Lung tissue, Human Mammary Cancer tissue. ICC/IF: U20S cell. Flow: Hela cell, PC-3 cell.
Target Information
The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy, a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARSA-I; ARSA1; Arylsulfatase A; ASA; ASNA-I; ATPase ASNA1; Cerebroside-sulfatase; EC 3.1.6.8; GET3; hASNA-I; TRC40
Gene Aliases: ARSA; As-2; AS-A; As2; ASA; AW212749; MLD; TISP73
UniProt ID: (Human) P15289, (Mouse) P50428
Entrez Gene ID: (Human) 410, (Rat) 315222, (Mouse) 11883
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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