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Proteintech

ASGR1 Polyclonal Antibody

View all (29) ASGR1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (13)

Application

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

ASGR1 Antibody (11739-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 11739-1-AP) ASGR1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ASGR1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

ASGR1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

ASGR1 Antibody in Western Blot (WB)

ASGR1 Antibody in Western Blot (WB)

ASGR1 Antibody in Western Blot (WB)

ASGR1 Antibody in Western Blot (WB)

ASGR1 Antibody in Flow Cytometry (Flow)

ASGR1 Antibody in Immunoprecipitation (IP)

ASGR1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 11739-1-AP) ASGR1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 11739-1-AP) ASGR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 11739-1-AP ( ASGR1 Antibody) at dilution of 1:200 (under 40x lens).

ASGR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 11739-1-AP ( ASGR1 Antibody) at dilution of 1:200 (under 10x lens).

ASGR1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 11739-1-AP (ASGR1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ASGR1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 11739-1-AP (ASGR1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ASGR1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ASGR1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11739-1-AP ( ASGR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ASGR1 Antibody in Western Blot (WB) — Human liver tissue were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ASGR1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ASGR1 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug ASGR1 antibody (11739-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 0.3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

ASGR1 Antibody in Immunoprecipitation (IP) — IP result of anti-ASGR1 (IP:11739-1-AP, 4 ug; Detection:11739-1-AP 1:2000) with mouse liver tissue lysate 4000 ug.

ASGR1 Antibody in Immunoprecipitation (IP) — IP result of anti-ASGR1 (IP:11739-1-AP, 4ug; Detection:11739-1-AP 1:500) with mouse liver tissue lysate 4000 ug.

Product Details

11739-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

0.2 µg/1x10^6 cells

-

Immunocytochemistry (ICC/IF)

-

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ASGR1 Fusion Protein Ag2310 (1-291 aa encoded by BC032130)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTKEYQDLQ HLDNEESDHH QLRKGPPPPQ PLLQRLCSGP RLLLLSLGLS LLLLVVVCVI GSQNSQLQEE LRGLRETFSN FTASTEAQVK GLSTQGGNVG RKMKSLESQL EKQQKDLSED HSSLLLHVKQ FVSDLRSLSC QMAALQGNGS ERTCCPVNWV EHERSCYWFS RSGKAWADAD NYCRLEDAHL VVVTSWEEQK FVQHHIGPVN TWMGLHDQNG PWKWVDGTDY ETGFKNWRPE QPDDWYGHGL GGGEDCAHFT DDGRWNDDVC QRPYRWVCET ELDKASQEPP LL (1-291 aa encoded by BC032130)

Target Information

The asialoglycooprotein receptor (ASGPR) is a transmembrane glycoprotein (42 kDa) which mediates binding, internalization and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i. e. it is present on the sinusoidal and lateral plasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediate the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed. The rat asialoglycoprotein receptor (ASGPR) consists of three polypeptide subunits: rat hepatic lectin (RHL)-1, RHL-2 and RHL-3. The monoclonal antibody 8D7 recognizes a subunit-specific epitope of RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ASGR1 promotes liver injury in sepsis by modulating monocyte-to-macrophage differentiation via NF-κB/ATF5 pathway.

Life sciences

Shi R,Wang J,Zhang Z,Leng Y,Chen AF

Wed Feb 15 00:00:00 UTC 2023

ASGR1 promotes liver injury in sepsis by modulating monocyte-to-macrophage differentiation via NF-κB/ATF5 pathway.

Life sciences

Shi R,Wang J,Zhang Z,Leng Y,Chen AF

Wed Feb 15 00:00:00 UTC 2023

ASGR1 promotes liver injury in sepsis by modulating monocyte-to-macrophage differentiation via NF-κB/ATF5 pathway.

Life sciences

Shi R,Wang J,Zhang Z,Leng Y,Chen AF

Wed Feb 15 00:00:00 UTC 2023

ASGR1 promotes liver injury in sepsis by modulating monocyte-to-macrophage differentiation via NF-κB/ATF5 pathway.

Life sciences

Shi R,Wang J,Zhang Z,Leng Y,Chen AF

Wed Feb 15 00:00:00 UTC 2023

ASGR1 promotes liver injury in sepsis by modulating monocyte-to-macrophage differentiation via NF-κB/ATF5 pathway.

Life sciences

Shi R,Wang J,Zhang Z,Leng Y,Chen AF

Wed Feb 15 00:00:00 UTC 2023

ASGR1 promotes liver injury in sepsis by modulating monocyte-to-macrophage differentiation via NF-κB/ATF5 pathway.

Life sciences

Shi R,Wang J,Zhang Z,Leng Y,Chen AF

Wed Feb 15 00:00:00 UTC 2023

Liver cancer cells as the model for developing liver-targeted RNAi therapeutics.

Biochemical and biophysical research communications

Hou B,Qin L,Huang L

Sun Feb 12 00:00:00 UTC 2023

Decellularized Liver Nanofibers Enhance and Stabilize the Long-Term Functions of Primary Human Hepatocytes In Vitro.

Advanced healthcare materials

Liu JS,Madruga LYC,Yuan Y,Kipper MJ,Khetani SR

Sat Jul 01 00:00:00 UTC 2023

Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion.

Nature

Wang JQ,Li LL,Hu A,Deng G,Wei J,Li YF,Liu YB,Lu XY,Qiu ZP,Shi XJ,Zhao X,Luo J,Song BL

Mon Aug 01 00:00:00 UTC 2022

ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth.

Cancer research

Ma WK,Voss DM,Scharner J,Costa ASH,Lin KT,Jeon HY,Wilkinson JE,Jackson M,Rigo F,Bennett CF,Krainer AR

Tue Mar 01 00:00:00 UTC 2022

Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.

Biomaterials

Jiang T,Xu L,Zhao M,Kong F,Lu X,Tang C,Yin C

Sat Jan 01 00:00:00 UTC 2022

Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.

Acta biomaterialia

Du K,Li S,Li C,Li P,Miao C,Luo T,Qiu B,Ding W

Fri Oct 15 00:00:00 UTC 2021

Neuraminidases 1 and 3 Trigger Atherosclerosis by Desialylating Low-Density Lipoproteins and Increasing Their Uptake by Macrophages.

Journal of the American Heart Association

Demina EP,Smutova V,Pan X,Fougerat A,Guo T,Zou C,Chakraberty R,Snarr BD,Shiao TC,Roy R,Orekhov AN,Miyagi T,Laffargue M,Sheppard DC,Cairo CW,Pshezhetsky AV

Tue Feb 16 00:00:00 UTC 2021

Neuraminidases 1 and 3 Trigger Atherosclerosis by Desialylating Low-Density Lipoproteins and Increasing Their Uptake by Macrophages.

Journal of the American Heart Association

Demina EP,Smutova V,Pan X,Fougerat A,Guo T,Zou C,Chakraberty R,Snarr BD,Shiao TC,Roy R,Orekhov AN,Miyagi T,Laffargue M,Sheppard DC,Cairo CW,Pshezhetsky AV

Tue Feb 16 00:00:00 UTC 2021

Regulated control of gene therapies by drug-induced splicing.

Nature

Monteys AM,Hundley AA,Ranum PT,Tecedor L,Muehlmatt A,Lim E,Lukashev D,Sivasankaran R,Davidson BL

Sun Aug 01 00:00:00 UTC 2021

Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood.

iScience

Matsumoto A,Takahashi Y,Ogata K,Kitamura S,Nakagawa N,Yamamoto A,Ishihama Y,Takakura Y

Fri Aug 20 00:00:00 UTC 2021

A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo.

Nano letters

Hajj KA,Melamed JR,Chaudhary N,Lamson NG,Ball RL,Yerneni SS,Whitehead KA

Wed Jul 08 00:00:00 UTC 2020

Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models.

Molecular therapy : the journal of the American Society of Gene Therapy

Kim Y,Jo M,Schmidt J,Luo X,Prakash TP,Zhou T,Klein S,Xiao X,Post N,Yin Z,MacLeod AR

Wed Sep 04 00:00:00 UTC 2019

Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models.

Molecular therapy : the journal of the American Society of Gene Therapy

Kim Y,Jo M,Schmidt J,Luo X,Prakash TP,Zhou T,Klein S,Xiao X,Post N,Yin Z,MacLeod AR

Wed Sep 04 00:00:00 UTC 2019

Delivery of GalNAc-Conjugated Splice-Switching ASOs to Non-hepatic Cells through Ectopic Expression of Asialoglycoprotein Receptor.

Molecular therapy. Nucleic acids

Scharner J,Qi S,Rigo F,Bennett CF,Krainer AR

Fri Jun 07 00:00:00 UTC 2019

Delivery of GalNAc-Conjugated Splice-Switching ASOs to Non-hepatic Cells through Ectopic Expression of Asialoglycoprotein Receptor.

Molecular therapy. Nucleic acids

Scharner J,Qi S,Rigo F,Bennett CF,Krainer AR

Fri Jun 07 00:00:00 UTC 2019

Characterization of Cellular Sources and Circulating Levels of Extracellular Vesicles in a Dietary Murine Model of Nonalcoholic Steatohepatitis.

Hepatology communications

Li J,Liu H,Mauer AS,Lucien F,Raiter A,Bandla H,Mounajjed T,Yin Z,Glaser KJ,Yin M,Malhi H

Sun Sep 01 00:00:00 UTC 2019

The FUT2 secretor variant p.Trp154Ter influences serum vitamin B12 concentration via holo-haptocorrin, but not holo-transcobalamin, and is associated with haptocorrin glycosylation.

Human molecular genetics

Velkova A,Diaz JEL,Pangilinan F,Molloy AM,Mills JL,Shane B,Sanchez E,Cunningham C,McNulty H,Cropp CD,Bailey-Wilson JE,Wilson AF,Brody LC

Fri Dec 15 00:00:00 UTC 2017

The FUT2 secretor variant p.Trp154Ter influences serum vitamin B12 concentration via holo-haptocorrin, but not holo-transcobalamin, and is associated with haptocorrin glycosylation.

Human molecular genetics

Velkova A,Diaz JEL,Pangilinan F,Molloy AM,Mills JL,Shane B,Sanchez E,Cunningham C,McNulty H,Cropp CD,Bailey-Wilson JE,Wilson AF,Brody LC

Fri Dec 15 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: ASGP-R 1; ASGPR 1; asialoglycoprotein receptor (RHL1); Asialoglycoprotein receptor 1; Asialoglycoprotein receptor 1 (hepatic lectin); asialoglycoprotein receptor RHL1; C-type lectin domain family 4 member H1; Hepatic lectin 1; hepatic lectin 1 RHL1; hepatic lectin 1, RHL1; Hepatic lectin H1; HL-1; RHL-1

Gene Aliases: ASGPR; ASGPR1; ASGR; Asgr-1; ASGR1; CLEC4H1; HL-1; RATRHL1; RHL1

UniProt ID: (Human) P07306, (Rat) P02706, (Mouse) P34927

Entrez Gene ID: (Human) 432, (Rat) 24210, (Mouse) 11889

Function(s)

asialoglycoprotein receptor activity protein binding carbohydrate binding metal ion binding protein homodimerization activity membrane traffic protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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