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ATAT1 Antibody (PA5-114922) in ICC/IF
Immunocytochemistry analysis of ATAT1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114922) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114922
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATAT1(Accession Q5SQI0), corresponding to amino acid residues E229-R279.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899558
Product Specific Information
Antibody detects endogenous levels of total C6orf134.
Target Information
This gene encodes a protein that localizes to clathrin-coated pits, where it acetylates alpha tubulin on lysine 40. This process may be important in microtubule growth, for instance during chemotaxis and the formation of cilium. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyltransferase mec-17 homolog; acetyltransferase mec-17 homolog {ECO:0000255|HAMAP-Rule:MF_03130}; Alpha-TAT; alpha-TAT {ECO:0000255|HAMAP-Rule:MF_03130}; alpha-TAT1; alpha-TAT1 {ECO:0000255|HAMAP-Rule:MF_03130}; Alpha-tubulin N-acetyltransferase 1; alpha-tubulin N-acetyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_03130}; Novel DUF738 containing protein (2610110G12Rik); TAT; TAT {ECO:0000255|HAMAP-Rule:MF_03130}
Gene Aliases: 0610011P08Rik; 2610008K08Rik; 2610110G12Rik; 3110080J08Rik; alpha-TAT; alpha-TAT1; ATAT1; C6orf134; MEC17; Nbla00487; RGD1303066; TAT
UniProt ID: (Human) Q5SQI0, (Mouse) Q8K341, (Rat) Q6MG11
Entrez Gene ID: (Human) 79969, (Mouse) 73242, (Rat) 361789
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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